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Enzymatic Synthesis of Anandamide, an Endogenous Cannabinoid Receptor Ligand, through N-Acylphosphatidylethanolamine Pathway in Testis: Involvement of Ca2+-Dependent Transacylase and Phosphodiesterase Activities

1996; Elsevier BV; Volume: 218; Issue: 1 Linguagem: Inglês

10.1006/bbrc.1996.0020

1090-2104

Takayuki Sugiura, Sachiko Kondo, Akihiro Sukagawa, Takashi Tonegawa, Shinji Nakane, Atsushi Yamashita, Keizo Waku,

Diet, Metabolism, and Disease

Rat testis was shown to contain significant amounts of both N-acylethanolamine, including N-arachidonoylethanolamine (anandamide), and N-acylphosphatidylethanolamine (N-acylPE), including N-arachidonoylPE. The fatty acid profiles of the N-acyl moieties of the two classes resembled each other. We confirmed that testis microsomes contain a phosphodiesterase activity catalyzing the release of anandamide from N-arachidonoylPE. They also contain an enzyme activity catalyzing the transfer of arachidonic acid from the 1-position of diacylphospholipids to PE to form N-arachidonoylPE. These results suggest that the N-acylPE pathway is important in the synthesis of anandamide in this tissue.