Separation of complex long chain fatty acid mixtures by high-performance glass capillary gas chromatography
1981; Elsevier BV; Volume: 216; Linguagem: Inglês
10.1016/s0021-9673(00)82340-7
ISSN1873-3778
AutoresLeslie Sisfontes, Göran Nyborg, Lennart Svensson, Rolf Blomstrand,
Tópico(s)Mass Spectrometry Techniques and Applications
ResumoDetailed descriptions are given for the preparation of high resolution open tubular glass capillary columns using SP-1000, Silar-5 CP and Silar-10 C as stationary phases. A comparison has been made between HCl etched soda lime glass capillaries and HCl leached soda lime or borosilicate glass capillaries with BaCO3 deposition. The best results were obtained with Silar-5 CP coated on HCl leached borosilicate capillaries with BaCO3 deposition. These types of columns have been used for the separation of complex long chain fatty acid mixtures including positional and geometrical isomers. The technique recommended has been used for 3 years and can easily be reproduced. A modification of the all-glass solvent-less injection system is also described. The qualitative and quantitative features of the glass capillary gas chromatography system are discussed.
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