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Role of glutathione peroxidase against oxidative stress in yeast: phenotypic character of lipid hydroperoxide-sensitive mutants derived from Hansenula mrakii

1993; Elsevier BV; Volume: 75; Issue: 3 Linguagem: Inglês

10.1016/0922-338x(93)90123-p

1872-8073

Yoshiharu Inoue, Linh-Thuoc Tran, Akira Kimura,

Fungal and yeast genetics research

Abstract Wild type cells of Hansenula mrakii IFO 0895 were highly resistant to the oxidative stress caused by lipid hydroperoxide. The resistance was due to a glutathione peroxidase (GSHPx) which was induced when the yeast was cultured in a medium containing lipid hydroperoxide (Inoue, Y. et al. , Agric. Biol. Chem., 54, 3289–3293, 1990). In order to investigate the role of GSHPx, two mutants sensitive to lipid hydroperoxide were isolated. The phenotypes of the mutants were temperature-dependent; i.e. , the mutants could grow at 28°C, but not at 35°C in the presence of lipid hydroperoxide. The mutants failed to induce the GSHPx at 35°C. However, the enzyme induced at 28°C and prepared from both mutants was stable after incubation at 37°C for 1 h.