Hydroxyapatite as a liquid chromatographic packing
1991; Elsevier BV; Volume: 544; Linguagem: Inglês
10.1016/s0021-9673(01)83984-4
ISSN1873-3778
Autores Tópico(s)Alkaline Phosphatase Research Studies
ResumoA general review of both classical and recent research on hydroxyapatite chromatography is presented, with the main purpose of establishing the specific mechanism involved in this type of chromatography. The deductions were confirmed with the aid of a crystallographic study of hydroxyapatite, adsorption experiments and quantitative analysis on the basis of a general theory of gradient chromatography. The mutual separation of proteins and other biomacromolecules with subtle structural differences is highly specific to hydroxyapatite chromatography.
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