Bovine Semen Metabolism. IV. Effect of Added Hormones and Amino Acids on Fructolysis
1959; Elsevier BV; Volume: 42; Issue: 1 Linguagem: Inglês
10.3168/jds.s0022-0302(59)90526-0
ISSN1529-9066
Autores Tópico(s)Reproductive Physiology in Livestock
ResumoA total of 46 semen samples was used to determine the effects of the addition of the hormones, epinephrine, insulin, thyroxine, and triiodothyronine, and of the sulfhydry]containing amino acids, ergothioneine and cysteine, on fructolysis in bull semen.A control tube and three tubes with the selected levels of compound addition and semen were incubated at 37 ° C. in a 2.9% sodium citrate buffer, and fructose utilizations were determined at 60 and 120 rain.Epinephrine addition (4-100 ~g. per milliliter) resulted in an inhibition of fruetolysis during the 0-to 60-min.period, with a return to the control level during the 60-to 120-rain.period.There was no apparent effect of insulin (4-100 ~g. per milliliter) ; thyroxine (0.04-1.00 ~g. per milliliter) ; triiodothyronine (0.008-0.200 ~g. per milliliter); ergothioneine (4-100 /xg.per milliliter), or cysteine (4-100 ~g. per milliliter) on fructose utilization.
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