Does endogenous cyclic GMP inhibit potassium stimulated 45Ca uptake by P2 fraction from rat brain?
1981; Elsevier BV; Volume: 29; Issue: 9 Linguagem: Inglês
10.1016/0024-3205(81)90399-4
ISSN1879-0631
AutoresSeiji Ichida, Takeshi Osugi, Hiroshi Yoshida,
Tópico(s)Neuropeptides and Animal Physiology
ResumoThe effects of sodium azide (NaN3), hydroxylamine (NH2OH) and sodium nitroprusside on potassium-stimulated 45Ca uptake (K-stimulated 45Ca uptake) by P2 fraction of Gray and Whittaker from rat brain were investigated. During preincubation with these reagents, the contents of cyclic GMP in synaptosomes increased, reaching maximum levels within 2 min. On preincubation for 2 min, NaN3, an activator of membrane bound guanylate cyclase, inhibited K-stimulated 45Ca uptake, but NH2OH and sodium nitroprusside did not affect it. Sodium cyanide, another metabolic inhibitor, had no effect on K-stimulated 45Ca uptake. There was a correlation between inhibition of K-stimulated 45Ca uptake and increase in the cGMP level on preincubation with NaN3 for various periods. Based on these results role of cGMP in or around the membrane was discussed in relation to the K-stimulated 45Ca uptake by P2 fraction.
Referência(s)