Continuous column denitrfication using polyelectrolyte complex-entrapped Paracoccus denitrificans cells
1987; Elsevier BV; Volume: 65; Issue: 3 Linguagem: Inglês
10.1016/0385-6380(87)90101-4
ISSN1878-2108
AutoresEtsuo Kokufuta, Masato Shimohashi, Isei Nakamura,
Tópico(s)Membrane Separation Technologies
ResumoAbstract Whole cells from Paracoccus denitrificans IFO 12442 were immobilized with a polyelectrolyte complex consisting of poly(vinyl alcohol) sulfate and poly(diallyldimethylammonium chloride). Continuous denitrification using a column packed with the immobilized cells was carried out as follows: A non-growth medium containing KNO3 as a substrate and potassium aspartate as a carbon source was passed through the column under anaerobic conditions for about three months. It was found that the immobilized system remained stable during a period of more than two months, without the loss of activity or the leakage of the cells from the support.
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