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Viral Ski Inhibits Retinoblastoma Protein (Rb)-mediated Transcriptional Repression in a Dominant Negative Fashion

1999; Elsevier BV; Volume: 274; Issue: 8 Linguagem: Inglês

10.1074/jbc.274.8.4485

1083-351X

Fumino Tokitou, Teruaki Nomura, Md Matiullah Khan, Sunil C. Kaul, Renu Wadhwa, Takashi Yasukawa, Isao Kohno, Shunsuke Ishii,

interferon and immune responses

The mechanism by which the viral oncogene ski (v-ski) transforms chicken embryo fibroblasts is currently unknown. Recently, the c-ski gene product (c-Ski) was found to bind to N-CoR (nuclear hormone receptor co-repressor), an element implicated in transcriptional repression mediated by multiple transcriptional repressors including the nuclear hormone receptors and Mad. c-Ski is required for transcriptional repression mediated by Mad involved in negative regulation of cellular proliferation. v-Ski abrogates Mad-induced transcriptional repression in a dominant negative fashion. Here we report that v-Ski also inhibits transcriptional repression mediated by Rb, another tumor suppressor gene product. Rb forms a complex with c-Ski, Sin3A, and histone deacetylase (HDAC) via direct binding to c-Ski and HDAC. c-Ski is required for the transcriptional repression mediated by Rb. These results suggest that inhibition of Rb activity contributes, at least partly, to transformation by v-Ski. The mechanism by which the viral oncogene ski (v-ski) transforms chicken embryo fibroblasts is currently unknown. Recently, the c-ski gene product (c-Ski) was found to bind to N-CoR (nuclear hormone receptor co-repressor), an element implicated in transcriptional repression mediated by multiple transcriptional repressors including the nuclear hormone receptors and Mad. c-Ski is required for transcriptional repression mediated by Mad involved in negative regulation of cellular proliferation. v-Ski abrogates Mad-induced transcriptional repression in a dominant negative fashion. Here we report that v-Ski also inhibits transcriptional repression mediated by Rb, another tumor suppressor gene product. Rb forms a complex with c-Ski, Sin3A, and histone deacetylase (HDAC) via direct binding to c-Ski and HDAC. c-Ski is required for the transcriptional repression mediated by Rb. These results suggest that inhibition of Rb activity contributes, at least partly, to transformation by v-Ski. The oncogene v-ski was originally identified in avian Sloan-Kettering viruses and found to transform chicken embryo fibroblasts (1Li Y. Turck C.M. Teumer J.K. Stavnezer E. J. Virol. 1986; 57: 1065-1072Crossref PubMed Google Scholar). The human c-ski 1The abbreviations c-skicellular ski genec-Skic-ski gene productHDAChistone deacetylaseN-CoRnuclear hormone receptor co-repressorRbretinoblastoma gene productv-Skiviralski gene productsnoski-related novel genePBSphosphate-buffered salineGSTglutathioneS-transferase proto-oncogene product (c-Ski) is a 728-amino acid nuclear protein, and the N- and C-terminal regions of c-Ski possess a cysteine-rich and a coiled-coil region, respectively (2Nomura N. Sasamoto S. Ishii S. Date T. Matsui M. Ishizaki R. Nucleic Acids Res. 1989; 17: 5489-5500Crossref PubMed Scopus (149) Google Scholar, 3Nagase T. Mizuguchi G. Nomura N. Ishizaki T. Ueno Y. Ishii S. Nucleic Acids Res. 1990; 18: 337-343Crossref PubMed Scopus (52) Google Scholar). The v-Ski protein lacks a 292-amino acid region from the C terminus of c-Ski, but still contains the N-proximal cysteine-rich region (4Stavnezer E. Brodeur D. Brennan L.A. Mol. Cell. Biol. 1989; 9: 4038-4045Crossref PubMed Scopus (56) Google Scholar). This N-proximal region is responsible for the cellular transformation capacity of ski(5Zheng G. Teumer J. Colmenares C. Richmond C. Stavnezer E. Oncogene. 1997; 15: 459-471Crossref PubMed Scopus (45) Google Scholar). The ski gene family comprises two members,ski and sno(s ki-related novel gene) (2Nomura N. Sasamoto S. Ishii S. Date T. Matsui M. Ishizaki R. Nucleic Acids Res. 1989; 17: 5489-5500Crossref PubMed Scopus (149) Google Scholar), and both have been shown to share clear homology in their N- and C-terminal regions (2Nomura N. Sasamoto S. Ishii S. Date T. Matsui M. Ishizaki R. Nucleic Acids Res. 1989; 17: 5489-5500Crossref PubMed Scopus (149) Google Scholar, 6Nagase T. Nomura N. Ishii S. J. Biol. Chem. 1993; 268: 13710-13716Abstract Full Text PDF PubMed Google Scholar). Recently we found that c-Ski directly binds to N-CoR (nuclear hormone receptor co-repressor) (28Nomura T. Khan M.M. Kaul S.C. Dong H.-D. Wadhwa R. Colmenares C. Ishii S. Genes Dev. 1999; (in press)PubMed Google Scholar). N-CoR was originally identified as a co-repressor that binds to and mediates transcriptional repression by nuclear hormone receptors (8Hörlein A.J. Näär A.M. Heinzel T. Torchia J. Gloss B. Kurokawa R. Ryan A. Kamei Y. Söderström M. Glass C.K. Rosenfeld M.G. Nature. 1995; 377: 397-404Crossref PubMed Scopus (1714) Google Scholar). Another co-repressor, SMRT, shows striking homology to N-CoR (9Chen J.D. Evans R.M. Nature. 1995; 377: 454-457Crossref PubMed Scopus (1715) Google Scholar). N-CoR also forms a complex with mammalian Sin3 orthologues (mSin3A and mSin3B). The binding of mSins to histone deacetylase (HDAC) suggested that transcriptional repression through N-CoR involves deacetylation of nucleosomal histones (10Heinzel T. Lavinsky R.M. Mullen T.-M. Söderström M. Laherty C.D. Torchia J. Yang W.-M. Brard G. Ngo S.D. Davie J.R. Seto E. Eisenman R.N. Rose D.W. Glass C.K. Rosenfeld M.G. Nature. 1997; 387: 43-48Crossref PubMed Scopus (1086) Google Scholar, 11Alland L. Muhle R. Hou Jr., H. Potes J. Chin L. Schreiber-Agus N. DePinho R.A. Nature. 1997; 387: 49-55Crossref PubMed Scopus (739) Google Scholar, 12Hassing C.A. Fleischer T.C. Billin A.N. Schreiber S.L. Ayer D.E. Cell. 1997; 89: 341-347Abstract Full Text Full Text PDF PubMed Scopus (661) Google Scholar, 13Laherty C.D. Yang W.-M. Sun J.-M. Davie J.R. Seto E. Eisenman R.N. Cell. 1997; 89: 349-356Abstract Full Text Full Text PDF PubMed Scopus (851) Google Scholar, 14Nagy L. Kao H.-Y. Chakravarti D. Lin R.J. Hassig C.A. Ayer D.E. Schreiber S.L. Evans R.M. Cell. 1997; 89: 373-380Abstract Full Text Full Text PDF PubMed Scopus (1110) Google Scholar). The basic helix-loop-helix proteins of the Mad family act as transcriptional repressors after heterodimerization with Max (15Ayer D.E. Kretzner L. Eisenman R.N. Cell. 1993; 72: 211-222Abstract Full Text PDF PubMed Scopus (623) Google Scholar). Mad interacts with the HDAC complex through direct binding to mSin3, and N-CoR is required for Mad-induced transcriptional repression (10Heinzel T. Lavinsky R.M. Mullen T.-M. Söderström M. Laherty C.D. Torchia J. Yang W.-M. Brard G. Ngo S.D. Davie J.R. Seto E. Eisenman R.N. Rose D.W. Glass C.K. Rosenfeld M.G. Nature. 1997; 387: 43-48Crossref PubMed Scopus (1086) Google Scholar, 11Alland L. Muhle R. Hou Jr., H. Potes J. Chin L. Schreiber-Agus N. DePinho R.A. Nature. 1997; 387: 49-55Crossref PubMed Scopus (739) Google Scholar, 12Hassing C.A. Fleischer T.C. Billin A.N. Schreiber S.L. Ayer D.E. Cell. 1997; 89: 341-347Abstract Full Text Full Text PDF PubMed Scopus (661) Google Scholar, 13Laherty C.D. Yang W.-M. Sun J.-M. Davie J.R. Seto E. Eisenman R.N. Cell. 1997; 89: 349-356Abstract Full Text Full Text PDF PubMed Scopus (851) Google Scholar, 14Nagy L. Kao H.-Y. Chakravarti D. Lin R.J. Hassig C.A. Ayer D.E. Schreiber S.L. Evans R.M. Cell. 1997; 89: 373-380Abstract Full Text Full Text PDF PubMed Scopus (1110) Google Scholar). We demonstrated that N-CoR binds to the N-terminal region of c-Ski and that this interaction is also required for transcriptional repression mediated by Mad and the thyroid hormone receptor β (28Nomura T. Khan M.M. Kaul S.C. Dong H.-D. Wadhwa R. Colmenares C. Ishii S. Genes Dev. 1999; (in press)PubMed Google Scholar). The same target sequence of Mad/Max, the so-called E-box, is also recognized by a heterodimer of Myc/Max that activates transcription. It is believed that Myc/Max enhances cellular proliferation or transformation, whereas Mad/Max leads to suppression of proliferation or induction of terminal differentiation in a wide range of cell types (16Ayer D.E. Eisenman R.N. Genes Dev. 1993; 7: 2110-2119Crossref PubMed Scopus (260) Google Scholar, 17Roussel M.F. Ashmun R.A. Sher C.J. Eisenman R.N. Ayer D.E. Mol. Cell. Biol. 1996; 16: 2796-2801Crossref PubMed Scopus (112) Google Scholar). Our data indicated that v-Ski blocks Mad-induced transcriptional repression in a dominant negative fashion (28Nomura T. Khan M.M. Kaul S.C. Dong H.-D. Wadhwa R. Colmenares C. Ishii S. Genes Dev. 1999; (in press)PubMed Google Scholar), suggesting that inhibition of Mad function contributes to transformation by v-Ski. cellular ski gene c-ski gene product histone deacetylase nuclear hormone receptor co-repressor retinoblastoma gene product viralski gene product ski-related novel gene phosphate-buffered saline glutathioneS-transferase In addition to Mad, the retinoblastoma protein (Rb) encoded by another tumor suppressor gene also binds to HDAC (18Brehm A. Miska E.A. McCance D.J. Reid J.L. Bannister A.J. Kouzarides T. Nature. 1998; 391: 597-601Crossref PubMed Scopus (1080) Google Scholar, 19Magnaghi-Jaulin L. Groisman R. Naguibneva I. Robin P. Lorain S. Le Villain J.P. Troalen F. Trouche D. Harel-Bellan A. Nature. 1998; 391: 601-605Crossref PubMed Scopus (805) Google Scholar, 20Luo R.X. Postigo A.A. Dean D.C. Cell. 1998; 92: 463-473Abstract Full Text Full Text PDF PubMed Scopus (839) Google Scholar). Rb regulates the G1/S transition in the cell cycle by silencing a group of target genes regulated by E2F transcription factors (21Weinberg R.A. Cell. 1995; 81: 323-330Abstract Full Text PDF PubMed Scopus (4326) Google Scholar, 22Nevins J.R. Science. 1992; 258: 424-429Crossref PubMed Scopus (1364) Google Scholar). Rb binds to the activation domain of E2F and then actively represses the promoter by recruiting HDAC. The pocket region of Rb, which contains two subdomains, termed A and B, are responsible for interaction with HDAC (18Brehm A. Miska E.A. McCance D.J. Reid J.L. Bannister A.J. Kouzarides T. Nature. 1998; 391: 597-601Crossref PubMed Scopus (1080) Google Scholar, 19Magnaghi-Jaulin L. Groisman R. Naguibneva I. Robin P. Lorain S. Le Villain J.P. Troalen F. Trouche D. Harel-Bellan A. Nature. 1998; 391: 601-605Crossref PubMed Scopus (805) Google Scholar, 20Luo R.X. Postigo A.A. Dean D.C. Cell. 1998; 92: 463-473Abstract Full Text Full Text PDF PubMed Scopus (839) Google Scholar). Although HDAC forms a complex with mSin3, N-CoR, and Ski, it remains unknown whether Rb can form a complex with any of these components of the N-CoR complex. To understand the molecular mechanism of transformation by v-Ski, we examined whether c-Ski forms a complex with Rb and whether v-Ski abrogates Rb-induced transcriptional activation as in the case of Mad. Our results indicate that c-Ski is needed for the transcriptional repression mediated by Rb and that v-Ski abrogates Rb-induced transcriptional repression. HeLa cells were lysed in lysis buffer consisting of PBS, 0.1% Nonidet P-40, 10% glycerol, and protease inhibitor mixture (Boehringer Mannheim). CV-1 cells were lysed in lysis buffer consisting of PBS, 1 mm NaF, 1 mm Na3VO4, 0.1% Nonidet P-40, 1 mm phenylmethylsulfonyl fluoride, 10% glycerol, and protease inhibitor mixture (Boehringer Mannheim). Lysates were immunoprecipitated using anti-c-Ski monoclonal antibodies (28Nomura T. Khan M.M. Kaul S.C. Dong H.-D. Wadhwa R. Colmenares C. Ishii S. Genes Dev. 1999; (in press)PubMed Google Scholar), anti-Sno monoclonal antibodies, anti-Rb antibody G3-245 or XZ91 (Pharmingen), anti-Gal4 antibody (Santa Cruz Biotechnology Inc.), or control IgG, and the immune complex was analyzed by Western blotting using the anti-Rb, anti-N-CoR (28Nomura T. Khan M.M. Kaul S.C. Dong H.-D. Wadhwa R. Colmenares C. Ishii S. Genes Dev. 1999; (in press)PubMed Google Scholar), anti-c-Ski, anti-mSin3A (Santa Cruz Biotechnology Inc.), or anti-HDAC1 (Santa Cruz Biotechnology Inc.) antibodies and ECL detection reagents (Amersham Pharmacia Biotech). Anti-c-Ski and anti-Sno monoclonal antibodies were prepared using bacterially expressed full-length c-Ski and Sno proteins, respectively. To examine the effect of v-Ski on complex formation among Rb, HDAC1, and Ski, CV-1 cells were transfected with a mixture of 1 μg of the c-Ski or v-Ski expression plasmid, 3 μg of the Gal4-Rb expression plasmid, and 1 μg of the HDAC1 expression plasmid using LipofectAMINETM (Life Technologies Inc.). The plasmid to express Gal4-Rb containing the DNA-binding domain of Gal4 (amino acids 1–174) and the repressor domain of Rb (amino acids 379–792) was constructed using the cytomegalovirus promoter-containing vector pcDNA3 (Invitrogen). The HDAC1 expression plasmid was constructed using pcDNA3, and the c-Ski and v-Ski expression plasmids were described (28Nomura T. Khan M.M. Kaul S.C. Dong H.-D. Wadhwa R. Colmenares C. Ishii S. Genes Dev. 1999; (in press)PubMed Google Scholar). Lysates were prepared and immunoprecipitated using anti-Gal4 antibody (UBI) or control IgG as described above, and the immune complex was analyzed by Western blotting using the anti-HDAC1 antibody (Santa Cruz Biotechnology Inc.). To express the GST-Rb fusion protein containing the pocket region of human Rb (amino acids 372–787) inEscherichia coli, a plasmid was constructed by the polymerase chain reaction-based method using the pGEX-2T vector (Amersham Pharmacia Biotech). The modified pSP65 vector pSPUTK (Stratagene) was used for in vitro transcription/translation of the various forms of Rb and c-Ski. The various deletion mutants of c-Ski have been described previously (28Nomura T. Khan M.M. Kaul S.C. Dong H.-D. Wadhwa R. Colmenares C. Ishii S. Genes Dev. 1999; (in press)PubMed Google Scholar), and the Rb deletion mutants were constructed using appropriate enzyme sites. The Rb mutants contained deletions of one of the following regions; ΔB (amino acids 600–928), ΔA (amino acids 302–600), ΔA + B (amino acids 414–928), or A + B (amino acids 1–371 and amino acids 789–928). The preparation of GST fusion proteins, the in vitro translation of various forms of Rb and c-Ski, and the binding assays were done essentially as described (28Nomura T. Khan M.M. Kaul S.C. Dong H.-D. Wadhwa R. Colmenares C. Ishii S. Genes Dev. 1999; (in press)PubMed Google Scholar) except for the use of a binding buffer consisting of 10 mm Hepes (pH 7.6), 0.1 m KCl, 2.5 mm MgCl2, 0.5 mmdithiothreitol, 0.05% Nonidet P-40, and 0.1 mg/ml bovine serum albumin and the use of PBS for washing. The antibody injection experiments using the Gal4-Rb expression plasmid and the anti-c-Ski and anti-Sno polyclonal antibodies were performed as described (28Nomura T. Khan M.M. Kaul S.C. Dong H.-D. Wadhwa R. Colmenares C. Ishii S. Genes Dev. 1999; (in press)PubMed Google Scholar). To investigate the effect of v-Ski on Gal4-Rb-mediated transcriptional repression, a mixture of 3 μg of the Gal site-containing luciferase reporter, 0.02 μg of Gal4-Rb or the Gal4 expression plasmid, and 1, 2, or 3 μg of the plasmid to express c-Ski or v-Ski and 0.5 μg of internal control plasmid pRL-TK was transfected into CV-1 cells using the CaPO4 method, and luciferase assays were performed. The total amount of plasmid DNA was adjusted to 15 μg by addition of the control plasmid DNA lacking the cDNA. To examine the effect of Ski on E2F-dependent transcriptional activation, a mixture of 0.1 μg of the E2F1 site-containing luciferase reporter, 0.05 μg of the E2F1 expression plasmid or the control DNA, and 1.5, 2, or 2.5 μg of the v-Ski expression plasmid and 0.1 μg of the internal control plasmid pRL-TK was transfected into CV-1 cells using LipofectAMINETM (Life Technologies, Inc.), and luciferase assays were performed. The total amount of plasmid DNA was adjusted to 3 μg by addition of the control plasmid DNA lacking the cDNA. The E2F site-containing reporter was described previously (23Ohtani K. Nevins J.R. Mol. Cell. Biol. 1994; 14: 1603-1612Crossref PubMed Scopus (111) Google Scholar). The pcDNA1 vector (Invitrogen) containing the cytomegalovirus promoter was used to express E2F1. To investigate whether Rb forms a complex with c-Ski in vivo, co-immunoprecipitation assays were performed (Fig.1 A). The cell lysates were prepared from HeLa cells and immunoprecipitated with anti-c-Ski, anti-Sno, or control anti-Gal4 antibody. Rb was co-precipitated with anti-c-Ski or anti-Sno antibodies but not with the control anti-Gal4 antibody. To examine complex formation between the endogenous Rb protein and mSin3A, co-immunoprecipitation was performed using CV-1 cells. The anti-Rb antibody XZ91 co-immunoprecipitated mSin3A, whereas the anti-Rb antibody G3-245 or control IgG did not (Fig.1 B). These results indicate that Rb forms a complex in vivo not only with HDAC but also with c-Ski and mSin3A. We could not detect N-CoR or SMRT in the complex immunoprecipitated with anti-Rb antibodies (data not shown). However, we cannot exclude the possibility that this is due to the low level of expression of N-CoR and SMRT in HeLa and CV-1 cells. To examine whether expression of v-Ski results in loss of HDAC in the Rb-HDAC complex, we performed the co-immunoprecipitation experiment (Fig. 1 C). CV-1 cells were transfected with a mixture of the c-Ski or v-Ski expression plasmid together with the plasmids to express HDAC1 and the Gal4-Rb fusion protein made up of the Gal4 DNA-binding domain and the pocket region of Rb. The cell lysates were prepared and immunoprecipitated with anti-Gal4 or control IgG antibody. The amount of HDAC1 coprecipitated with Gal4-Rb in the presence of v-Ski was apparently less than that with c-Ski. These results suggest that v-Ski inhibits association between Rb and HDAC1 in a dominant negative fashion. During the analysis of the interaction between Rb and the components of the N-CoR complex, we found that c-Ski directly binds to Rb in vitro. In the GST pull-down assays using in vitrotranslated Rb and the GST-c-Ski resin containing full-length c-Ski, a significant amount of in vitro translated Rb was found to bind to the GST-c-Ski resin (Fig.2 A). The results of binding assays using the different mutants of Rb indicated that the B subdomain in the pocket region of Rb is responsible for the interaction with c-Ski. To identify the region in c-Ski that interacts with Rb, the GST pull-down assay was performed using the GST-Rb fusion protein resin and various forms of in vitro translated c-Ski protein (Fig.2 B). The results indicated that two regions in c-Ski efficiently interact with Rb; one is the region between amino acids 197 and 330, which includes a part of the N-CoR-binding domain (amino acids 99–274) and the other is the C-terminal coiled-coil region (amino acids 556–728). Thus, Rb directly binds to c-Ski and HDAC. To further investigate whether c-Ski is required for transcriptional repression by Rb, antibody injection experiments were done (Fig.3). Injection into Rat-1 cells of alacZ reporter plasmid containing the lacZ gene linked to the TK promoter and Gal4-binding sites gave rise to manylacZ-positive cells. Co-injection of this lacZreporter with a plasmid encoding the Gal4-Rb fusion protein made up of the Gal4 DNA-binding domain and the pocket region of Rb resulted in a decrease in the number of lacZ-positive cells. This decrease was relieved significantly by co-injection of anti-c-Ski antibody and partially by anti-Sno antibodies. Co-injection of both antibodies also significantly relieved the decrease in the number oflacZ-positive cells, but not completely. The incomplete abrogation of Gal-Rb function by co-injection of both antibodies may be due to the presence of other Ski-related protein(s) such as the third member of the ski gene family which we identified recently. 2M. M. Khan, T. Nomura, and S. Ishii, unpublished data. As a control experiment, we used a Gal4 fusion protein containing the repressor domain of δEF1, which is thought not to utilize the N-CoR c-Ski complex. Co-injection of anti-c-Ski or anti-Sno antibodies did not alleviate the decrease in the number of lacZ-positive cells induced by Gal4-δEF1 (28Nomura T. Khan M.M. Kaul S.C. Dong H.-D. Wadhwa R. Colmenares C. Ishii S. Genes Dev. 1999; (in press)PubMed Google Scholar), indicating that the effect of anti-Ski/Sno antibodies was specific for Rb. To investigate whether v-Ski mutants that lack the C-terminal region of c-Ski could abrogate transcriptional repression by Rb in a dominant negative fashion as in the case of Mad, we examined the effect of overexpression of v-Ski on Gal4-Rb-induced transcriptional repression (Fig. 4, A and B). Gal4-Rb containing the pocket region of Rb strongly repressed transcription from the Gal4 site-containing reporter. This Gal4-Rb-induced repression was abrogated by v-Ski in a dose-dependent manner. Furthermore, wild type c-Ski partly abrogated Gal4-Rb-induced transcriptional repression. We observed that the microspeckle pattern of N-CoR was disrupted by coexpression of a high amount of c-Ski but not by a low amount of c-Ski. 3T. Nomura and S. Ishii, unpublished data. These two observations are consistent with the idea that overexpression of wild type c-Ski abrogates transcriptional repression by creating an imbalance between the components of the co-repressor complex rather than potentiating transcriptional repression. In control experiments, repression by the Gal4-δEF1 fusion protein was not abolished by co-expression of either v-Ski or wild type c-Ski (28Nomura T. Khan M.M. Kaul S.C. Dong H.-D. Wadhwa R. Colmenares C. Ishii S. Genes Dev. 1999; (in press)PubMed Google Scholar). Using the E2F1 site-containing luciferase reporter, we also examined the effect of v-Ski on E2F1-mediated transcriptional activation (Fig. 4 C). v-Ski was also found to enhance E2F1-induced transcriptional activation in a dose-dependent manner. These results indicate that v-Ski inhibits Rb-dependent transcriptional repression. The oncogene v-ski can transform chicken embryo fibroblasts. Our results indicate that v-Ski abrogates transcriptional repression mediated not only by Mad but also by Rb. c-Ski has two regions that are conserved in related proteins, the N-terminal cysteine-rich region and the C-terminal coiled-coil region. N-CoR binds to the N-terminal cysteine-rich region, while the C-terminal coiled-coil region binds to mSin3 (28Nomura T. Khan M.M. Kaul S.C. Dong H.-D. Wadhwa R. Colmenares C. Ishii S. Genes Dev. 1999; (in press)PubMed Google Scholar). The C-truncated c-Ski protein lacking the coiled-coil region cannot bind to mSin3 and disrupts the dot-like structure of N-CoR (28Nomura T. Khan M.M. Kaul S.C. Dong H.-D. Wadhwa R. Colmenares C. Ishii S. Genes Dev. 1999; (in press)PubMed Google Scholar), suggesting that this form of c-Ski acts as in a dominant negative fashion. Because v-Ski also lacks the C-terminal coiled-coil region, v-Ski probably inhibits Mad- and Rb-mediated transcriptional repression in a dominant negative fashion. In our co-transfection assay, overexpression of normal c-Ski also partly abrogated the transcriptional repression mediated by Rb (Fig.4). This is consistent with the fact that overexpression of wild type c-Ski also leads to transformation (24Colmenares C. Sutrave P. Hughes S.H. Stavnezer E. J. Virol. 1991; 65: 4929-4935Crossref PubMed Google Scholar). Mutation of the human Rb gene occurs in a wide variety of tumors (25Goodrich D.W. Lee W.H. Biochim. Biophys. Acta. 1993; 1155: 43-61Crossref PubMed Scopus (156) Google Scholar). In addition, one of themad-related genes, mxi1, was recently demonstrated to act as a tumor suppressor using mutant mice (26Schreiber-Agus N. Meng Y. Hoang T. Hou Jr., H. Chen K. Greenberg R. Cordon-Cardo C. Lee H.-W. DePinho R.A. Nature. 1998; 393: 483-487Crossref PubMed Scopus (173) Google Scholar). Therefore, abrogation of Rb and Mad activity by v-Ski may contribute, at least partly, to transformation by v-ski. Rb was recently reported to directly bind to HDAC (18Brehm A. Miska E.A. McCance D.J. Reid J.L. Bannister A.J. Kouzarides T. Nature. 1998; 391: 597-601Crossref PubMed Scopus (1080) Google Scholar, 19Magnaghi-Jaulin L. Groisman R. Naguibneva I. Robin P. Lorain S. Le Villain J.P. Troalen F. Trouche D. Harel-Bellan A. Nature. 1998; 391: 601-605Crossref PubMed Scopus (805) Google Scholar, 20Luo R.X. Postigo A.A. Dean D.C. Cell. 1998; 92: 463-473Abstract Full Text Full Text PDF PubMed Scopus (839) Google Scholar). Our results indicate that Rb also directly interacts with c-Ski. Furthermore, Rb forms a complex with mSin3, although it is not clear whether the Rb-HDAC-mSin3A-Ski complex contains N-CoR. The antibody injection experiments showed that c-Ski is required for Rb-mediated transcriptional repression (Fig. 3). At present, it remains unknown whether N-CoR and mSin3 are needed for the transcriptional repression mediated by Rb. Thus, c-Ski is required for the transcriptional repression mediated by at least Mad, thyroid receptor, and Rb. It is possible that other transcriptional repressors that utilize the N-CoR-mSin3-HDAC complex also require c-Ski. The complex containing mSin3 consists of multiple proteins such as SAP30 and the histone-binding proteins RbAp46 and RbAp48 (27Zhang Y. Iratni R. Erdjument-Bromage H. Tempst P. Reinberg D. Cell. 1997; 89: 357-364Abstract Full Text Full Text PDF PubMed Scopus (504) Google Scholar). Interestingly, SAP30 is required for the transcriptional repression mediated by the estrogen receptor but not by thyroid receptor or the retinoic acid receptor (7Laherty C.D. Billin A.N. Lavinsky R.M. Yochum G.S. Bush A.C. Sun J.-M. Mullen T.-M. Davie J.R. Rose D.W. Glass C.K. Rosenfeld M.G. Ayer D.E. Eisenman R.N. Mol. Cell. 1998; 2: 33-42Abstract Full Text Full Text PDF PubMed Scopus (186) Google Scholar). To understand the molecular mechanism of v-Ski-induced transformation, it will be important to determine whether c-Ski acts in specific transcriptional repression mediated by a limited number of repressors or in transcriptional repression in general. We thank Dr. T. Dryja for the human Rb cDNA, Drs. K. Ohtani and M. Ikeda for the E2F site-containing luciferase reporter and the E2F1 expression plasmid, and Dr. S. L. Schreiber for the HDAC1 cDNA.