Release of cytochrome c from mitochondria to cytosol in gerbil hippocampal CA1 neurons after transient forebrain ischemia
1999; Elsevier BV; Volume: 849; Issue: 1-2 Linguagem: Inglês
10.1016/s0006-8993(99)01971-x
ISSN1872-6240
AutoresHiroki Nakatsuka, Shinsuke Ohta, Junya Tanaka, Kazuko Toku, Yoshiaki Kumon, Nobuji Maeda, Masahiro Sakanaka, Saburo Sakaki,
Tópico(s)Alzheimer's disease research and treatments
ResumoWe examined cytosolic cytochrome c in gerbil hippocampal CA1 and CA3 regions after induction of 5-min ischemia by immunoblotting. In the CA1 region, cytochrome c was detected in the cytosolic fraction from 1 to 6 h after ischemia by Western blotting, while it was not detected in the CA3 region. Following intraventricular administration of cyclosporin A (CsA), detectable cytosolic cytochrome c was dramatically decreased, and about 80% of CA1 neurons survived after ischemia. The present studies demonstrate that cytochrome c is translocated from mitochondria to the cytosol in the early stage of delayed neuronal cell death, and suggest the involvement of the mitochondrial permeability transition.
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