Characterization ofp53 gene mutations in basal-cell carcinomas: Comparison between sun-exposed and less-exposed skin areas
1996; Wiley; Volume: 65; Issue: 6 Linguagem: Inglês
10.1002/(sici)1097-0215(19960315)65
ISSN1097-0215
AutoresYasuhiro Matsumura, Chikako Nishigori, Takashi Yagi, Sadao Imamura, Hiraku Takebe,
Tópico(s)Cancer-related Molecular Pathways
ResumoMutations in the p53 gene in 32 basal-cell carcinomas (BCC) developed in Japanese patients were identified by the polymerase chain reaction and single-strand-conformation polymorphism analysis, followed by sequencing the DNA. Among 16 BCC developed in continuously sun-exposed areas, 6 tumors showed 7 base substitutions, most of which were G:C to A:T transitions, mainly at the dipyrimidine sites. Seven out of 16 BCC developed in less-exposed areas showed 8 base substitutions, but the majority (75.0%) of them were transversions. These results suggest that the mutation in the p53 gene plays a significant role in the tumorigenesis of BCC developed in less-exposed areas as well as those in sun-exposed areas in Japanese patients. There must be therefore causative factors other than UV irradiation for BCC in less-exposed areas. © 1996 Wiley-Liss, Inc.
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