Cell-specific protein profiling in Arabidopsis thaliana trichomes: identification of trichome-located proteins involved in sulfur metabolism and detoxification
2004; Elsevier BV; Volume: 65; Issue: 11 Linguagem: Inglês
10.1016/j.phytochem.2004.03.026
ISSN1873-3700
AutoresStefanie Wienkoop, Daniela Zoeller, Berit Ebert, Ulrike Simon-Rosin, Joachim Fisahn, Mirko Glinski, Wolfram Weckwerth,
Tópico(s)Molecular Biology Techniques and Applications
ResumoMetabolite, protein, and transcript analysis at the cellular level gives unparalleled insight into the complex roles tissues play in the plant system. However, while capillary electrophoresis and PCR amplification strategies make the profiling of metabolites and transcripts in specific cell types possible, the profiling of proteins in small samples represents a bottleneck. Here for the first time protein profiling has been achieved in a specific plant cell type: The application of specific cell sampling and shotgun peptide sequencing (nano LC/MS/MS) resulted in the identification of 63 unique proteins from pooled Arabidopsis trichome cells. A complete S-adenosylmethionine pathway cluster, two S-adenosylmethionine synthase isoforms, a glutathione S-conjugate translocator and other proteins involved in sulfur metabolism and detoxification are shown to be present in these cells, in agreement with previous work done at the level of trichome transcript analysis. The technology described here brings the simultaneous identification and localization of physiologically relevant cellular proteins within reach.
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