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Constitutive arsenite oxidase expression detected in arsenic-hypertolerant Pseudomonas xanthomarina S11

2015; Elsevier BV; Volume: 166; Issue: 3 Linguagem: Inglês

10.1016/j.resmic.2015.02.010

1769-7123

Sandrine Koechler, Florence Arsène‐Ploetze, Céline Brochier‐Armanet, Florence Goulhen-Chollet, Audrey Heinrich-Salmeron, Bernard Jost, Didier Lièvremont, Muriel Philipps, Frédéric Plewniak, Philippe Bertin, Marie-Claire Lett,

Chromium effects and bioremediation

Pseudomonas xanthomarina S11 is an arsenite-oxidizing bacterium isolated from an arsenic-contaminated former gold mine in Salsigne, France. This bacterium showed high resistance to arsenite and was able to oxidize arsenite to arsenate at concentrations up to 42.72 mM As[III]. The genome of this strain was sequenced and revealed the presence of three ars clusters. One of them is located on a plasmid and is organized as an “arsenic island” harbouring an aio operon and genes involved in phosphorous metabolism, in addition to the ars genes. Neither the aioXRS genes nor a specific sigma-54-dependent promoter located upstream of aioBA genes, both involved in regulation of arsenite oxidase expression in other arsenite-oxidizing bacteria, could be identified in the genome. This observation is in accordance with the fact that no difference was observed in expression of arsenite oxidase in P. xanthomarina S11, whether or not the strain was grown in the presence of As[III].