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PHYLOGENY OF THE RABBIT γ‐CHAIN DETERMINANTS: A d12‐LIKE ANTIGENIC DETERMINANT IN PRONOLAGUS RUPESTRIS

1979; Wiley; Volume: 6; Issue: 6 Linguagem: Inglês

10.1111/j.1744-313x.1979.tb00692.x

1744-313X

C. Hamers‐Casterman, E. Wittouck, Wessel van der Loo, R. Hamers,

Protein purification and stability

Summary A survey for constant region γ‐chain allotypes (de locus) was undertaken in different lagomorph genera. As yet only Pronolagus rupestris , a paleolaginae, showed the presence of a determinant similar to rabbit d12 although it lacked the widespread e15 determinant. All seven individuals possessed the d12 like determinant which was studied by immunodiffusion, haemagglutination inhibition, radiobinding and binding inhibition assays. In addition, a new enzymic method for typing for d12, based on the presence of the asymmetric rabbit hing carbohydrate linked to the d12 characteristic threonine, is presented. This method suggests, however, that, unlike the d12 rabbit, Pronolagus does not seem to have a majority of IgG molecules with a glycosylated hinge.