Portal de Periódicos da CAPES

The development of the action potential mechanism of amphibian neurons isolated in culture.

1976; National Academy of Sciences; Volume: 73; Issue: 5 Linguagem: Inglês

10.1073/pnas.73.5.1641

1091-6490

Nicholas C. Spitzer, Janet E. Lamborghini,

Photoreceptor and optogenetics research

Nerve and muscle cells differentiated morphologically, in cultures of dissociated cells prepared from amphibian neural plate and underlying mesoderm (Xenopus laevis, Nieuwkoop and Faber stage 15). Cultures were grown in a defined medium containing sterile Steinberg's salt solution and 0.1% bovine serum albumin, and maintained for periods up to 5 days.