Portal de Periódicos da CAPES

Dark Quenched Matrix Metalloproteinase Fluorogenic Probe for Imaging Osteoarthritis Development in Vivo

2008; American Chemical Society; Volume: 19; Issue: 9 Linguagem: Inglês

10.1021/bc800264z

1520-4812

Seulki Lee, Kyeongsoon Park, Seung Young Lee, Ju Hee Ryu, Jong Woong Park, Hyung Jun Ahn, Ick Chan Kwon, Inchan Youn, Kwangmeyung Kim, Kuiwon Choi,

Inflammatory mediators and NSAID effects

The early detection of osteoarthritis (OA) is currently a key challenge in the field of rheumatology. Biochemical studies of OA have indicated that matrix metalloproteinase-13 (MMP-13) plays a central role in cartilage degradation. In this study, we describe the potential use of a dark-quenched fluorogenic MMP-13 probe to image MMP-13 in both in vitro and rat models. The imaging technique involved using a MMP-13 peptide substrate, near-infrared (NIR) dye, and a NIR dark quencher. The results from this study demonstrate that the use of a dark-quenched fluorogenic probe allows for the visual detection of MMP-13 in vitro and in OA-induced rat models. In particular, by targeting this OA biomarker, the symptoms of the early and late stages of OA can be readily monitored, imaged, and analyzed in a rapid and efficient fashion. We anticipate that this simple and highly efficient fluorogenic probe will assist in the clinical management of patients with OA, not only for early diagnosis but also to assess individual patient responses to new drug treatments.