Transplantation of rabbit kidneys perfused with glycerol solutions at 10 °C
1978; Elsevier BV; Volume: 15; Issue: 1 Linguagem: Inglês
10.1016/0011-2240(78)90003-2
ISSN1090-2392
AutoresIb Abildgaard Jacobsen, E. Pegg, Monica C. Wusteman, Simon Robinson,
Tópico(s)Metabolism and Genetic Disorders
ResumoExperiments are described in which rabbit kidneys were perfused with solutions containing 2, 3, or 4 m glycerol and then were autografted in order to measure any functional impairment. The basic perfusate contained Haemaccel (17.5 g/litre) and mannitol (111 mm) and had a total osmolality of 400 mosmol/kg. The perfusion temperature was 10 °C. Glycerol concentration was slowly increased at a rate of 30 mm min−1 to 2, 3, or 4 m, was held at the maximum concentration for 30 min, and was then decreased to < 0.1 m at 30 mm min−1. An immediate contralateral nephrectomy was carried out, and the function of the perfused kidney was assessed by serial measurements of blood urea and serum creatinine, by the determination of 24-hr endogenous creatinine clearance at 1 month and by histological examination. It was found that a concentration of 3 m glycerol was readily tolerated.
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