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Isolation and Identification of a New Bifidogenic Growth Stimulator Produced by Propionibacterium freudenreichii ET-3

2002; Oxford University Press; Volume: 66; Issue: 3 Linguagem: Inglês

10.1271/bbb.66.679

1347-6947

Kakuhei Isawa, Kenichi Hojo, Nobuo Yoda, Tomonori Kamiyama, Seiya Makino, Mizue SAITO, Hitomi SUGANO, Chinami Mizoguchi, Saori KURAMA, Mika SHIBASAKI, Noriko Endo, Yoshio Satō,

Microbial Metabolic Engineering and Bioproduction

We have found a new growth stimulator for bifidobacteria in the culture broth of Propionibacterium freudenreichii ET-3. The bifidogenic growth stimulator (BGS) was purified by Diaion HP-20 column chromatography and preparative HPLC. Spectroscopic methods including 1H-NMR, UV, and LC-ESI-MS experiments indicated that the chemical structure of the bifidogenic growth stimulator was 1,4-dihydroxy-2-naphthoic acid (DHNA). Approximately 10 mg/L of DHNA was found to be produced in the culture broth of P. freudenreichii ET-3.