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Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection

2011; Centers for Disease Control and Prevention; Volume: 17; Issue: 2 Linguagem: Inglês

10.3201/eid1702.101123

1080-6059

Cassandra E. Faux, Katherine E. Arden, Stephen B. Lambert, Michael D. Nissen, Terry Nolan, Anne B. Chang, Theo P. Sloots, Ian M Mackay,

Animal Disease Management and Epidemiology

Abstract We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)–specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR is required for accurate description of HRV epidemiology.