La phosphoglycolate phosphatase des feuilles de haricot: Deux formes isofonctionnelles
1979; Elsevier BV; Volume: 18; Issue: 8 Linguagem: Inglês
10.1016/0031-9422(79)83007-1
ISSN1873-3700
AutoresC. Vérin-Vergeau, P. Baldy, G. Cavalié,
Tópico(s)Algal biology and biofuel production
Resumo2-Phosphoglycollate phosphohydrolase (EC 3.1.3.18) was isolated and partially purified from leaves of Phaseolus vulgaris L. by acetone fractionation followed by chromatography on DEAE-cellulose. Two chromatographically stable forms could be separated by stepwise elution from DEAE-cellulose; they have been resolved by electrofocusing (pI 4.2 and 5.5) and exhibited two protein bands (Ritfs 0.68 and 0.82) on polyacrylamide gel electrophoresis. These data suggest the isoenzymatic nature of the two forms which are specific for phosphoglycollate.
Referência(s)