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Molecular identification of similar species of the genus Biomphalaria (Mollusca: Planorbidae) determined by a polymerase chain reaction-restriction fragment length polymorphism

1998; Instituto Oswaldo Cruz, Ministério da Saúde; Volume: 93; Issue: suppl 1 Linguagem: Inglês

10.1590/s0074-02761998000700039

1678-8060

Roberta Lima Caldeira, Teofânia HDA Vidigal, Sônia Torquato Paulinelli, Andrew J.G. Simpson, Omar S Carvalho,

Invertebrate Taxonomy and Ecology

The freshwater snails Biomphalaria straminea, B. intermedia, B. kuhniana and B. peregrina, are morphologically similar; based on this similarity the first three species were therefore grouped in the complex B. straminea.The morphological identification of these species is based on characters such as vaginal wrinkling, relation between prepuce: penial sheath:deferens vas and number of muscle layers in the penis wall.In this study the polymerase chain reaction restriction fragment length polymorphism technique was used for molecular identification of these molluscs.This technique is based on the amplification of the internal transcribed spacer regions ITS1 e ITS2 of the ribosomal RNA gene and subsequent digestion of these fragments by restriction enzymes.Six enzymes were tested: Dde I, Mnl I, Hae III, Rsa I, Hpa II e Alu I.The restriction patterns obtained with DdeI presented the best profile for separation of the four species of Biomphalaria.The profiles obtained with all the enzymes were used to estimate the genetic distances among the species through analysis of common banding patterns.