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Two-dimensional versus three-dimensional morphometry of monogenoidean sclerites

2007; Elsevier BV; Volume: 37; Issue: 3-4 Linguagem: Inglês

10.1016/j.ijpara.2006.11.017

1879-0135

Paolo Galli, Giovanni Strona, Anna Villa, Francesca Benzoni, Fabrizio Stefani, Silvia Maria Doglia, Delane C. Kritsky,

Marine Biology and Ecology Research

A new method of three-dimensional (3-D) analysis of sclerotised structures of monogenoids was performed by processing z-series images using 3D-Doctor. Z-series were obtained from Gomori’s trichrome-stained specimens of marine and freshwater monogenoids under laser scanning confocal fluorescence microscopy. Measurements obtained from 3-D images were then compared with those from 2-D images taken from both flattened and unflattened specimens. Data comparison demonstrated that 3-D morphometry allowed avoidance of over-estimation due to deformation and the reduction of errors associated with different spatial orientations. Moreover, study of 3-D images permitted observation of morphological details that are not detectable in 2-D representations.