Produção Nacional
Revisado por pares
Naturally acquired inhibitory antibodies to Plasmodium vivax Duffy binding protein are short-lived and allele-specific following a single malaria infection
2009; Oxford University Press; Volume: 156; Issue: 3 Linguagem: Inglês
10.1111/j.1365-2249.2009.03931.x
ISSN1365-2249
AutoresIsabela P. Cerávolo, Bruno Antônio Marinho Sanchez, Taís Nóbrega de Sousa, B. Guerra, Irene S. Soares, Érika Martins Braga, Amy M. McHenry, John H. Adams, Cristiana Ferreira Alves de Brito, Luzia H. Carvalho,
Tópico(s)Toxoplasma gondii Research Studies
ResumoSummary The Duffy binding protein of Plasmodium vivax (DBP) is a critical adhesion ligand that participates in merozoite invasion of human Duffy-positive erythrocytes. A small outbreak of P. vivax malaria, in a village located in a non-malarious area of Brazil, offered us an opportunity to investigate the DBP immune responses among individuals who had their first and brief exposure to malaria. Thirty-three individuals participated in the five cross-sectional surveys, 15 with confirmed P. vivax infection while residing in the outbreak area (cases) and 18 who had not experienced malaria (non-cases). In the present study, we found that only 20% (three of 15) of the individuals who experienced their first P. vivax infection developed an antibody response to DBP; a secondary boosting can be achieved with a recurrent P. vivax infection. DNA sequences from primary/recurrent P. vivax samples identified a single dbp allele among the samples from the outbreak area. To investigate inhibitory antibodies to the ligand domain of the DBP (cysteine-rich region II, DBPII), we performed in vitro assays with mammalian cells expressing DBPII sequences which were homologous or not to those from the outbreak isolate. In non-immune individuals, the results of a 12-month follow-up period provided evidence that naturally acquired inhibitory antibodies to DBPII are short-lived and biased towards a specific allele.
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