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Kinetics of Growth Retardant and Hormone Interactions in Affecting Cucumber Hypocotyl Elongation

1967; Oxford University Press; Volume: 42; Issue: 5 Linguagem: Inglês

10.1104/pp.42.5.677

1532-2548

Thomas C. Moore,

Plant Molecular Biology Research

The capacities of indole-3-acetic acid (IAA) and gibberellin A(3) (GA(3)) to counteract the inhibitory effects of (2-chloroethyl) trimethylammonium chloride (CCC), 2-isopropyl-4-dimethylamino-5-methylphenyl-1-piperidinecarboxylate methyl chloride (Amo-1618), and N,N-dimethylaminosuccinamic acid (B-995) on hypocotyl elongation in light-grown cucumber (Cucumis sativus L.) seedlings were investigated. One mug of GA(3) applied to the shoot tip was sufficient to completely nullify the effect of 10 mug of Amo-1618 or 25 mug of B-995 applied simultaneously to the shoot tip, and 10 mug of GA(3) completely counteracted the effect of 10(-3)m CCC added to the root medium. One mug of IAA counteracted the effect of 10(-3)m CCC in the root medium, but IAA did not nullify the action of either Amo-1618 or B-995. Experiments were conducted using 2 growth retardants simultaneously, which indicated that Amo-1618 and CCC inhibit a common process, namely GA biosynthesis, essential to hypocotyl elongation. However, since the effect of CCC was overcome by applications of both GA and IAA, growth retardation resulting from treatment with CCC apparently is not due solely to inhibition of GA biosynthesis. B-995 did not interact additively with either Amo-1618 or CCC, which suggests that B-995 affects a process different from those affected by the other 2 retardants. Thus, while inhibition evoked by B-995 is reversible by applied GA, the action of B-995 does not appear to be inhibition of GA biosynthesis.