Portal de Periódicos da CAPES

Simultaneous determination of nitroglycerin and its dinitrate metabolites by capillary gas chromatography with electron-capture detection

1988; Elsevier BV; Volume: 426; Linguagem: Inglês

10.1016/s0378-4347(00)81954-4

1872-812X

Frank W. Lee, Nobutoshi Watari, Jean F. Rigod, Leslie Z. Benet,

Analytical Chemistry and Chromatography

A sensitive gas chromatographic—electron-capture detection method for the simultaneous determination of the antianginal drug nitroglycerin (GTN) and its dinitrate metabolites (1,2-GDN and 1,3-GDN) was developed. Human plasma samples (1 ml) spiked with 2,6-dinitrotoluene as the internal standard were extracted once with 10 ml of a methylene chloride—pentane mixture (3:7, v/v). Using this solvent system, less contaminants are extracted into the organic phase from plasma, resulting in cleaner chromatograms and prolonged column life. A break point was observed on the standard curves of GTN and GDNs. The two linear regions for the detectable concentrations of GTN are 0.025–0.3 and 0.3–3 ng/ml and for 1,2-GDN and 1,3-GDN they are 0.1–1 and 1–10 ng/ml. The limits of detection by this method for GTN, 1,2-GDN and 1,3-GDN in plasma are 0.025, 0.1 and 0.1 ng/ml, respectively.