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Use of molecular markers for species identification of Korean Perkinsus sp. isolated from Manila clams Ruditapes philippinarum

2005; Inter-Research; Volume: 66; Linguagem: Inglês

10.3354/dao066255

1616-1580

KI Park, JK Park, Jehee Lee, Kyung Sook Choi,

Aquaculture disease management and microbiota

DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 66:255-263 (2005) - doi:10.3354/dao066255 Use of molecular markers for species identification of Korean Perkinsus sp. isolated from Manila clams Ruditapes philippinarum Kyung-Il Park1, Joong-Ki Park2, Jehee Lee1, Kwang-Sik Choi1,* 1School of Applied Marine Science, College of Ocean Science, Cheju National University, 1 Ara 1-Dong Jeju City,Jeju 690-756, Republic of Korea2Department of Parasitology and Medical Research Institute, College of Medicine, Chungbuk National University, Chongju,Chungbuk 361-763, Republic of Korea *Corresponding author. Email: skchoi@cheju.ac.kr ABSTRACT: Perkinsus is the pathogen responsible for mass mortality of the Manila clam Ruditapes philippinarum. Perkinsus sp. isolated from Manila clams collected in Korean waters was assayed by polymerase chain reaction (PCR) to determine its phylogenetic affinity with other congeneric species. Regions of rRNA of Perkinsus sp. isolated from clam haemolymph were cloned and sequenced. Sequences of a non-transcribed spacer (NTS), internal transcribed spacers (ITS 1, 2) and 5.8S rRNA genes were compared to those available from other Perkinsus species. The NTS sequence of Korean Perkinsus was approximately 99.9 to 100% similar to that of P. atlanticus and 98.06 to 98.15% and 73.05 to 73.14% similar to those of P. olseni and P. marinus, respectively. The ITS 1, 5.8S rRNA and ITS 2 sequences of Korean Perkinsus showed 100% similarity to P. atlanticus and Perkinsus sp. reported from Japan. The ITS–5.8S rRNA sequences of Korean Perkinsus were 99.86 and 93.73% similar to those of P. olseni and P. marinus, respectively. The sporulation pattern and morphology of the Korean Perkinsus were very similar to those of P. atlanticus. Our data suggest that the Perkinsus sp. isolated from clams in Korean waters is P. atlanticus, which is currently synonymous with P. olseni reported from Australia. By considering that P. olseni has taxonomic priority, Korean Perkinsus sp. is accepted as P. olseni (atlanticus). KEY WORDS: NTS · ITS · 5.8S rRNA · Perkinsus olseni (atlanticus) · Ruditapes philippinarum · Taxonomy · Korea Full text in pdf format PreviousNextExport citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 66, No. 3. Online publication date: September 23, 2005 Print ISSN: 0177-5103; Online ISSN: 1616-1580 Copyright © 2005 Inter-Research.