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Cardiac fibroblast to myofibroblast differentiation in vivo and in vitro: Expression of focal adhesion components in neonatal and adult rat ventricular myofibroblasts

2010; Wiley; Volume: 239; Issue: 6 Linguagem: Inglês

10.1002/dvdy.22280

1097-0177

Jon‐Jon Santiago, Aran L. Dangerfield, Sunil G. Rattan, Krista L. Bathe, Ryan H. Cunnington, Joshua E. Raizman, Kristen M. Bedosky, Darren H. Freed, Elissavet Kardami, Ian Dixon,

Electrospun Nanofibers in Biomedical Applications

Abstract In fibrosing hearts, myofibroblasts are associated with cardiac extracellular matrix remodeling. Expression of key genes in the transition of cardiac fibroblast to myofibroblast phenotype in post‐myocardial infarction heart and in vitro has not been well addressed. Contractile, focal adhesion‐associated, receptor proteins, fibroblast growth factor‐2 (FGF‐2) expression, and motility were compared to assess phenotype in adult and neonatal rat cardiac fibroblasts and myofibroblasts. Neonatal and adult fibroblasts undergo phenotypic transition to myofibroblastic cells, marked by increased α‐smooth muscle actin (αSMA), smooth muscle myosin heavy chain (SMemb), extra domain‐A (ED‐A) fibronectin, paxillin, tensin, FGF‐2, and TβRII receptor. Elevated ED‐A fibronectin confirmed fibroblast to supermature myofibroblastic phenotype transition. Presence of myofibroblasts in vivo was noted in sections of healed infarct scar after myocardial infarction, and their expression is similar to that in culture. Thus, cultured neonatal and adult cardiac fibroblasts transition to myofibroblasts in vitro and share expression profiles of cardiac myofibroblasts in vivo. Reduced motility with in vitro passage reflects enhanced production of focal adhesions. Developmental Dynamics 239:1573–1584, 2010. © 2010 Wiley‐Liss, Inc.