Fractionation and quantitative determination of urinary 17-hydroxycorticosteroids by thin layer chromatography on silica gel
1963; Elsevier BV; Volume: 1; Issue: 5 Linguagem: Inglês
10.1016/s0039-128x(63)80106-3
ISSN1878-5867
AutoresO. Adamec, Ján Matis, Marcel Galvánek,
Tópico(s)Steroid Chemistry and Biochemistry
ResumoA simple rapid method for the separation and quantitative determination of urinary 17-hydroxycorticosteroids is described. The urine is hydrolysed by means of beta-glucuronidase and extracted with chloroform. Both, the purification of the crude extract and the following separation of individual steroids are performed by chromatography on a thin layer of silica gel. For the purification, chromatography water is used as the mobile phase. The isolated mixture of corticsteroids is applied to a second chromatogram which is developed with 7%-ethanol in chloroform or 5%-ethanol in methylene chloride. The chromatogram is detected by spraying with tetrazolium blue. The sites corresponding to 17-hydroxycorticosteroids are exhausted, transferred into a test tube and subjected to colorimetry which is carried out by Portet — Silber reaction.
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