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Lipase-catalyzed Preparation of Phospholipids Containing n-3 Polyunsaturated Fatty Acids from Soy Phospholipids

2014; Japan Oil Chemists' Society; Volume: 63; Issue: 12 Linguagem: Inglês

10.5650/jos.ess14125

1347-3352

Y. Yamamoto, Eri Mizuta, Mizue Ito, Masataka Harata, Shigeru Hiramoto, Setsuko Hara,

Enzyme Catalysis and Immobilization

To utilize n-3 polyunsaturated fatty acid (PUFA) for a wide range of applications, we prepared phospholipids (PLs) containing PUFAs as constituent fatty acids (PUFA-PLs) via commercially available lipase OF-mediated transacylation with PL from soy (Soy-PL) and ethyl ester of PUFA (PUFA-Et). In a preliminary study to evaluate PUFA-incorporation (wt%) on phosphatidylcholine (PC), we observed that dehydration of Soy-PL is critical. PUFA-incorporation in PLs increased with acyl ratio and time. Finally, maximum PUFA-incorporation (47.1 ± 2.1 wt%) was obtained using the following reaction conditions: 2.0 mmol of Soy-PL, a PUFA-Et/Soy-PL acyl ratio of 7, 13 mL of hexane, 2.2 × 105 U of lipase OF, 500 rpm of agitation, a temperature of 37°C, and 72 h of reaction time. The analysis of fatty acid composition at the sn-2 position of obtained PL revealed that PUFAs incorporated into Soy-PL localized to the sn-2 position of the PL molecule in spite of using lipase OF whose positional specificity is random for triacylglycerol.