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Characteristic Features of Thermal Stability Map of DNA in Eschericia coli and Eukaryotic Genes

1988; Taylor & Francis; Volume: 6; Issue: 1 Linguagem: Inglês

10.1080/07391102.1988.10506482

1538-0254

Ryo Hanai, Akira Suyama, Akiyoshi Wada,

Bacterial Genetics and Biotechnology

Distribution of double-helix thermal stability of Escherichia coli and eukaryotic DNAs was analyzed. The results confirmed the previous propositions based on the study of the stability distribution in phage DNAs: (1) stability fluctuation appears near the boundaries of protein coding regions (PCRs) and non protein coding regions (NPCRs); (2) PCRs have less fluctuation than NPCRs. The present analysis also revealed that the local G + C content is lower in the beginning of PCRs of E. coli than the average G + C content of PCR and that deviations in the amino acid composition and the third letter usage PCRs are involved in the low G + C content; the biological meaning of this is discussed in relation to mRNA structure.