Immobilization of cytochrome P-450 and electrochemical control of its activity
1998; Wiley; Volume: 9; Issue: 5 Linguagem: Inglês
10.1002/(sici)1099-1581(199805)9
ISSN1099-1581
AutoresNobuhiro Sugihara, Yoshiro Ogoma, Kôji Abe, Yoshiyuki Kondo, Toshihiro Akaike,
Tópico(s)Electrochemical Analysis and Applications
ResumoPolymers for Advanced TechnologiesVolume 9, Issue 5 p. 307-313 Research Article Immobilization of cytochrome P-450 and electrochemical control of its activity Nobuhiro Sugihara, Nobuhiro Sugihara Department of Functional Polymer Science, Faculty of Textile Science and Technology, Shinshu University, Ueda 386, JapanSearch for more papers by this authorYoshiro Ogoma, Yoshiro Ogoma Department of Functional Polymer Science, Faculty of Textile Science and Technology, Shinshu University, Ueda 386, JapanSearch for more papers by this authorKoji Abe, Koji Abe Department of Functional Polymer Science, Faculty of Textile Science and Technology, Shinshu University, Ueda 386, JapanSearch for more papers by this authorYoshiyuki Kondo, Corresponding Author Yoshiyuki Kondo Department of Functional Polymer Science, Faculty of Textile Science and Technology, Shinshu University, Ueda 386, JapanDepartment of Functional Polymer Science, Faculty of Textile Science and Technology, Shinshu University, Ueda 386, JapanSearch for more papers by this authorToshihiro Akaike, Toshihiro Akaike Department of Biomolecular Engineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 277, JapanSearch for more papers by this author Nobuhiro Sugihara, Nobuhiro Sugihara Department of Functional Polymer Science, Faculty of Textile Science and Technology, Shinshu University, Ueda 386, JapanSearch for more papers by this authorYoshiro Ogoma, Yoshiro Ogoma Department of Functional Polymer Science, Faculty of Textile Science and Technology, Shinshu University, Ueda 386, JapanSearch for more papers by this authorKoji Abe, Koji Abe Department of Functional Polymer Science, Faculty of Textile Science and Technology, Shinshu University, Ueda 386, JapanSearch for more papers by this authorYoshiyuki Kondo, Corresponding Author Yoshiyuki Kondo Department of Functional Polymer Science, Faculty of Textile Science and Technology, Shinshu University, Ueda 386, JapanDepartment of Functional Polymer Science, Faculty of Textile Science and Technology, Shinshu University, Ueda 386, JapanSearch for more papers by this authorToshihiro Akaike, Toshihiro Akaike Department of Biomolecular Engineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 277, JapanSearch for more papers by this author First published: 04 December 1998 https://doi.org/10.1002/(SICI)1099-1581(199805)9:5 3.0.CO;2-1Citations: 21AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract P-450cam (camphor-induced cytochrome P-450) was immobilized on an indium tin oxide (ITO) electrode by polypyrrole and its activity was controlled electrochemically. The results showed that P-450cam was immobilized on the ITO electrode without denaturing and the amount of P-450cam could be easily controlled. When, the electric potential was swept repeatedly between 0.4 and 0 V, the remarkable decrease of oxygen in the reaction mixture solution was observed only in the presence of camphor. In addition, hydroxycamphor was detected only in the same system by means of gas chromatography/mass spectroscopy. These results suggested that immobilized P-450cam catalyzed the hydroxylation of camphor by the supply of electron from the electrode. The effect of pH and ionic strength on the activity was examined, and it was found that the high activity expressed at the pH of 6.0 – 7.0 and KCl concentration of 0.1 – 0.2 M. The paper is the first report that P-450 enzyme activity could be controlled artificially. © 1998 John Wiley & Sons, Ltd. Citing Literature Volume9, Issue5May 1998Pages 307-313 RelatedInformation
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