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Effects of CGS-15943A on the relaxations produced by adenosine analogs in human blood vessels

1990; Elsevier BV; Volume: 187; Issue: 3 Linguagem: Inglês

10.1016/0014-2999(90)90381-f

1879-0712

Majd H. Sabouni, George L. Brown, Alvin N. Kotake, Frank L. Douglas, S. Jamal Mustafa,

Nitric Oxide and Endothelin Effects

Abstract The antagonistic effects of CGS-15943A on the relaxations produced by adenosine and its analogs in human blood vessels were investigated in vitro. Donor hearts were the source of coronary arteries, whereas the internal mammary arteries and saphenous veins were obtained from patients undergoing coronary bypass surgery. Adenosine and its analogs, 5′-N-ethylcarboxamidoadenosine (NECA) and 2-chloroadenosine (CAD), produced concentration-dependent relaxations in KCl-contracted coronary rings. CGS-15943A antagonized, significantly, the relaxations produced by adenosine, NECA and CAD in coronary arteries. Similarly, the adenosine receptor antagonist, 8-phenyltheophylline (8PT, 10-μmol/1), caused a significant attenuation of the relaxing responses to adenosine, NECA and CAD in coronary arteries. In rings obtained from internal mammary arteries and saphenous veins, adenosine, NECA and CAD all produced concentration-dependent relaxations. These relaxations were smaller in magnitude than those obtained in coronary arteries, and were slightly greater in rings contracted with 10 μmol/1 prostaglandin F 2α (PGF 2α ) as compared to 35 mmol/1 KCl. However, the mammary arteries and saphenous veins relaxed completely in response to 100 μmol/1 papaverine. CGS-15943A (10 μmol/1) did not antagonize the relaxing effects of adenosine and its analogs in these vessels. The results show that coronary arteries are more responsive than mammary arteries or saphenous veins to the relaxing effects of adenosine analogs and that these relaxing responses are dependent on the contracting agent. Furthermore, CGS-15943A demonstrated antagonism of the adenosine response in coronary arteries.