Portal de Periódicos da CAPES

Response to fibronectin–integrin interaction in leukaemia cells: delayed enhancing of a K + current

1992; Royal Society; Volume: 248; Issue: 1323 Linguagem: Inglês

10.1098/rspb.1992.0067

1471-2954

Andrea Becchetti, Annarosa Arcangeli, Maria Riccarda Del Bene, Massimo Olivotto, Enzo Wanke,

Lipid Membrane Structure and Behavior

Restricted accessMoreSectionsView PDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmail Cite this article Becchetti Andrea , Arcangeli Annarosa , Del Bene Maria Riccarda , Olivotto Massimo and Wanke Enzo 1992Response to fibronectin–integrin interaction in leukaemia cells: delayed enhancing of a K+ currentProc. R. Soc. Lond. B.248235–240http://doi.org/10.1098/rspb.1992.0067SectionRestricted accessArticleResponse to fibronectin–integrin interaction in leukaemia cells: delayed enhancing of a K+ current Andrea Becchetti Google Scholar Find this author on PubMed Search for more papers by this author , Annarosa Arcangeli Google Scholar Find this author on PubMed Search for more papers by this author , Maria Riccarda Del Bene Google Scholar Find this author on PubMed Search for more papers by this author , Massimo Olivotto Google Scholar Find this author on PubMed Search for more papers by this author and Enzo Wanke Google Scholar Find this author on PubMed Search for more papers by this author Andrea Becchetti Google Scholar Find this author on PubMed , Annarosa Arcangeli Google Scholar Find this author on PubMed , Maria Riccarda Del Bene Google Scholar Find this author on PubMed , Massimo Olivotto Google Scholar Find this author on PubMed and Enzo Wanke Google Scholar Find this author on PubMed Published:22 June 1992https://doi.org/10.1098/rspb.1992.0067AbstractIn murine erythroleukaemia cells, the response of ion channels was followed before and after contact with fibronectin-coated latex microspheres. Patch-clamp experiments in 'whole-cell' and in 'cell-attached' configurations showed that cell adhesion to fibronectin promoted plasma membrane hyperpolarization mediated by activation of potassium channels that were indistinguishable from calcium-dependent potassium channels K(Ca) in these cells. K+ current increase began in 5-6 min and was completed about 10 min after the first contact. The timecourse of this process recorded from 'whole-cell' was very similar to that followed in intact cells by observing the increase of single channel currents. The open probability of single channels in the patch increased after contact, revealing that this activation is propagated at distance from the adhesion site. The slow onset of the effect suggests the presence of a complex regulatory pathway between fibronectin-integrin binding and activation of potassium channels. Decreasing cytoplasmic free Ca2+ concentration to pCa 9 diminished, but did not inhibit, the response. The current induced by fibronectin was not blocked by apamin, α-charybdotoxin or glibenclamide, but was abolished by high concentrations of tetraethylammonium (TEA). These data suggest for the first time the existence of a specific regulative connection between integrin receptors and ionic channels.FootnotesThis text was harvested from a scanned image of the original document using optical character recognition (OCR) software. As such, it may contain errors. Please contact the Royal Society if you find an error you would like to see corrected. Mathematical notations produced through Infty OCR. Previous ArticleNext Article VIEW FULL TEXT DOWNLOAD PDF FiguresRelatedReferencesDetailsCited by Ciriza J, Rodríguez-Romano A, Nogueroles I, Gallego-Ferrer G, Cabezuelo R, Pedraz J and Rico P (2021) Borax-loaded injectable alginate hydrogels promote muscle regeneration in vivo after an injury, Materials Science and Engineering: C, 10.1016/j.msec.2021.112003, 123, (112003), Online publication date: 1-Apr-2021. Rico P, Rodrigo-Navarro A, Sánchez Pérez L and Salmeron-Sanchez M (2020) Borax induces osteogenesis by stimulating NaBC1 transporter via activation of BMP pathway, Communications Biology, 10.1038/s42003-020-01449-4, 3:1, Online publication date: 1-Dec-2020. McLaughlin K and Levin M (2018) Bioelectric signaling in regeneration: Mechanisms of ionic controls of growth and form, Developmental Biology, 10.1016/j.ydbio.2017.08.032, 433:2, (177-189), Online publication date: 1-Jan-2018. Goligorsky M, Patschan D, Kuo M, Park H, Hochegger K, Rosenkranz A, Brady H and Mayadas T (2018) Cell Adhesion Molecules in Renal Injury Comprehensive Toxicology, 10.1016/B978-0-08-100601-6.00813-9, (507-532), . Becchetti A, Crescioli S, Zanieri F, Petroni G, Mercatelli R, Coppola S, Gasparoli L, D'Amico M, Pillozzi S, Crociani O, Stefanini M, Fiore A, Carraresi L, Morello V, Manoli S, Brizzi M, Ricci D, Rinaldi M, Masi A, Schmidt T, Quercioli F, Defilippi P and Arcangeli A (2017) The conformational state of hERG1 channels determines integrin association, downstream signaling, and cancer progression, Science Signaling, 10.1126/scisignal.aaf3236, 10:473, (eaaf3236), Online publication date: 4-Apr-2017. Brackenbury W (2016) Ion Channels in Cancer Ion Channels in Health and Disease, 10.1016/B978-0-12-802002-9.00006-6, (131-163), . Rooj A, Liu Z, McNicholas C and Fuller C (2015) Physical and functional interactions between a glioma cation channel and integrin-β 1 require α-actinin , American Journal of Physiology-Cell Physiology, 10.1152/ajpcell.00036.2015, 309:5, (C308-C319), Online publication date: 1-Sep-2015. Arcangeli A, Crociani O and Bencini L (2014) Interaction of tumour cells with their microenvironment: ion channels and cell adhesion molecules. A focus on pancreatic cancer, Philosophical Transactions of the Royal Society B: Biological Sciences, 369:1638, Online publication date: 19-Mar-2014. Crociani O, Becchetti A, Fanelli D and Arcangeli A (2014) Adhesion-Mediated Signalling in Cancer: Recent Advances and Mathematical Modelling, Biophysical Reviews and Letters, 10.1142/S1793048014300047, 09:03, (285-300), Online publication date: 1-Sep-2014. Shemarova I and Nesterov V (2013) Evolution of mechanisms of Ca2+-signalization. Role of Ca2+ in regulation of specialized cell functions, Journal of Evolutionary Biochemistry and Physiology, 10.1134/S0022093013010027, 49:1, (10-24), Online publication date: 1-Jan-2013. Arcangeli A (2011) Ion channels and transporters in cancer. 3. Ion channels in the tumor cell-microenvironment cross talk, American Journal of Physiology-Cell Physiology, 10.1152/ajpcell.00113.2011, 301:4, (C762-C771), Online publication date: 1-Oct-2011. Gui P, Chao J, Wu X, Yang Y, Davis G and Davis M (2010) Coordinated Regulation of Vascular Ca2+ and K+ Channels by Integrin Signaling Integrins and Ion Channels, 10.1007/978-1-4419-6066-5_7, (69-79), . Goligorsky M, Patschan D, Kuo M, Park H, Hochegger K, Rosenkranz A, Brady H and Mayadas T (2010) Cell Adhesion Molecules in Renal Injury Comprehensive Toxicology, 10.1016/B978-0-08-046884-6.00813-7, (213-244), . Arcangeli A and Becchetti A (2010) Integrin Structure and Functional Relation with Ion Channels Integrins and Ion Channels, 10.1007/978-1-4419-6066-5_1, (1-7), . Becchetti A and Arcangeli A (2008) A Comment on Ion Channels as Pharmacological Targets in Oncology, Journal of General Physiology, 10.1085/jgp.200810069, 132:2, (313-314), Online publication date: 1-Aug-2008. Wu X, Yang Y, Gui P, Sohma Y, Meininger G, Davis G, Braun A and Davis M (2008) Potentiation of large conductance, Ca 2+ -activated K + (BK) channels by α5β1 integrin activation in arteriolar smooth muscle , The Journal of Physiology, 10.1113/jphysiol.2007.149500, 586:6, (1699-1713), Online publication date: 15-Mar-2008. Pillozzi S, Brizzi M, Bernabei P, Bartolozzi B, Caporale R, Basile V, Boddi V, Pegoraro L, Becchetti A and Arcangeli A (2007) VEGFR-1 (FLT-1), β1 integrin, and hERG K+ channel for a macromolecular signaling complex in acute myeloid leukemia: role in cell migration and clinical outcome, Blood, 10.1182/blood-2006-02-003772, 110:4, (1238-1250), Online publication date: 15-Aug-2007. Kawasaki J, Davis G and Davis M (2004) Regulation of Ca2+-dependent K+ Current by αvβ3 Integrin Engagement in Vascular Endothelium, Journal of Biological Chemistry, 10.1074/jbc.M313791200, 279:13, (12959-12966), Online publication date: 1-Mar-2004. Vasilyev D and Barish M (2004) Regulation of the hyperpolarization-activated cationic current I h in mouse hippocampal pyramidal neurones by vitronectin, a component of extracellular matrix , The Journal of Physiology, 10.1113/jphysiol.2004.069104, 560:3, (659-675), Online publication date: 1-Nov-2004. Fraser S, Salvador V, Manning E, Mizal J, Altun S, Raza M, Berridge R and Djamgoz M (2003) Contribution of functional voltage-gated Na+ channel expression to cell behaviors involved in the metastatic cascade in rat prostate cancer: I. lateral motility, Journal of Cellular Physiology, 10.1002/jcp.10312, 195:3, (479-487), Online publication date: 1-Jun-2003. Vasilyev D and Barish M (2003) Regulation of an inactivating potassium current (IA) by the extracellular matrix protein vitronectin in embryonic mouse hippocampal neurones, The Journal of Physiology, 10.1113/jphysiol.2002.036889, 547:3, (859-871), Online publication date: 15-Mar-2003. Artym V and Petty H (2002) Molecular Proximity of Kv1.3 Voltage-gated Potassium Channels and β1-Integrins on the Plasma Membrane of Melanoma Cells, Journal of General Physiology, 10.1085/jgp.20028607, 120:1, (29-37), Online publication date: 1-Jul-2002. Hofmann G, Bernabei P, Crociani O, Cherubini A, Guasti L, Pillozzi S, Lastraioli E, Polvani S, Bartolozzi B, Solazzo V, Gragnani L, Defilippi P, Rosati B, Wanke E, Olivotto M and Arcangeli A (2001) HERG K+ Channels Activation during β1Integrin-mediated Adhesion to Fibronectin Induces an Up-regulation of αvβ3 Integrin in the Preosteoclastic Leukemia Cell Line FLG 29.1, Journal of Biological Chemistry, 10.1074/jbc.M005682200, 276:7, (4923-4931), Online publication date: 1-Feb-2001. Laniado M, Fraser S and Djamgoz M (2001) Voltage-gated K+ channel activity in human prostate cancer cell lines of markedly different metastatic potential: Distinguishing characteristics of PC-3 and LNCaP cells, The Prostate, 10.1002/1097-0045(20010301)46:4 3.0.CO;2-F, 46:4, (262-274), Online publication date: 1-Mar-2001. Levite M, Cahalon L, Peretz A, Hershkoviz R, Sobko A, Ariel A, Desai R, Attali B and Lider O (2000) Extracellular K+ and Opening of Voltage-Gated Potassium Channels Activate T Cell Integrin Function, Journal of Experimental Medicine, 10.1084/jem.191.7.1167, 191:7, (1167-1176), Online publication date: 3-Apr-2000. Bubien J, Keeton D, Fuller C, Gillespie G, Reddy A, Mapstone T and Benos D (1999) Malignant human gliomas express an amiloride-sensitive Na + conductance , American Journal of Physiology-Cell Physiology, 10.1152/ajpcell.1999.276.6.C1405, 276:6, (C1405-C1410), Online publication date: 1-Jun-1999. Colden-Stanfield M and Gallin E (1998) Modulation of K + currents in monocytes by VCAM-1 and E-selectin on activated human endothelium , American Journal of Physiology-Cell Physiology, 10.1152/ajpcell.1998.275.1.C267, 275:1, (C267-C277), Online publication date: 1-Jul-1998. Platts S, Mogford J, Davis M and Meininger G (1998) Role of K + channels in arteriolar vasodilation mediated by integrin interaction with RGD-containing peptide , American Journal of Physiology-Heart and Circulatory Physiology, 10.1152/ajpheart.1998.275.4.H1449, 275:4, (H1449-H1454), Online publication date: 1-Oct-1998. Grimes J and Djamgoz M (1998) Electrophysiological characterization of voltage-gated Na+ current expressed in the highly metastatic Mat-LyLu cell line of rat prostate cancer, Journal of Cellular Physiology, 10.1002/(SICI)1097-4652(199804)175:1 3.0.CO;2-B, 175:1, (50-58), Online publication date: 1-Apr-1998. Goligorsky M and Noiri E (1997) Role of NO in Cell Locomotion Nitric Oxide and the Kidney, 10.1007/978-1-4615-6039-5_5, (86-110), . Arcangel A, Faravelli L, Bianchi L, Rosati B, Gritti A, Vescovy A, Wanke E and Olivotto M (2009) Soluble or Bound Laminin Elicit in Human Neuroblastoma Cells Short- or Long-Term Potentiation of a K + Inwardly Rectifying Current: Relevance to Neuritogenesis , Cell Adhesion and Communication, 10.3109/15419069609010779, 4:4-5, (369-385), Online publication date: 1-Jan-1996. Covault J (1996) Cell Adhesion Molecular Biology of Membrane Transport Disorders, 10.1007/978-1-4613-1143-0_2, (11-45), . Sanders D, Thompson D, Philpott M, Kealey T, Fiddes I and Westgate G (1996) In the Absence of Streptomycin, Minoxidil Potentiates the Mitogenic Effects of Fetal Calf Serum, Insulin-Like Growth Factor 1, and Platelet-Derived Growth Factor on NIH 3T3 Fibroblasts in a K + Channel-Dependent Fashion, Journal of Investigative Dermatology, 10.1111/1523-1747.ep12329697, 107:2, (229-234), Online publication date: 1-Aug-1996. Noiri E, Romanov V, Forest T, Gailit J, DiBona G, Miller F, Som P, Oster Z and Goligorsky M (1995) Pathophysiology of renal tubular obstruction: Therapeutic role of synthetic RGD peptides in acute renal failure, Kidney International, 10.1038/ki.1995.426, 48:5, (1375-1385), Online publication date: 1-Nov-1995. Pethő Z, Najder K, Carvalho T, McMorrow R, Todesca L, Rugi M, Bulk E, Chan A, Löwik C, Reshkin S and Schwab A (2020) pH-Channeling in Cancer: How pH-Dependence of Cation Channels Shapes Cancer Pathophysiology, Cancers, 10.3390/cancers12092484, 12:9, (2484) This Issue22 June 1992Volume 248Issue 1323 Article InformationDOI:https://doi.org/10.1098/rspb.1992.0067PubMed:1354362Published by:Royal SocietyPrint ISSN:0962-8452Online ISSN:1471-2954History: Manuscript received25/02/1992Manuscript accepted23/03/1992Published online01/01/1997Published in print22/06/1992 License:Scanned images copyright © 2017, Royal Society Citations and impact Large datasets are available through Proceedings B's partnership with Dryad