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Immortalization of Normal Adult Human Middle Ear Epithelial Cells Using a Retrovirus Containing the E6/E7 Genes of Human Papillomavirus Type 16

2002; SAGE Publishing; Volume: 111; Issue: 6 Linguagem: Inglês

10.1177/000348940211100606

1943-572X

Sung‐Kyun Moon, Derald E. Brackmann, Young‐Myoung Chun, Haa-Yung Lee, Johng S. Rhim, Paul Webster, David Lim,

Herpesvirus Infections and Treatments

A human middle ear epithelial cell line (HMEEC-1) was established using human papillomavirus E6/E7 genes. HMEEC-1 has remained morphologically and phenotypically stable, even after 50 passages. The cells are anchorage-dependent and nontumorigenic when injected into nude mice. This cell line thus provides a new tool for the study of normal cell biology and the pathological processes associated with the epithelial cells of the middle ear in otitis media. HMEEC-1 will also be useful in the search for new drugs and biological agents for the treatment of otitis media.