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Cloning and molecular characterization of the citrate utilization citMCDEFGRP cluster of Leuconostoc paramesenteroides

1999; Oxford University Press; Volume: 174; Issue: 2 Linguagem: Inglês

10.1111/j.1574-6968.1999.tb13573.x

1574-6968

Mauricio Martı́n, Marı́a Angeles Corrales, Diego de Mendoza, Paloma Là pez, Christian Magni,

Microbial Metabolites in Food Biotechnology

The citMCDEFGRP cluster from Leuconostoc paramesenteroides involved in citrate utilization was cloned and its nucleotide sequence determined. Homology of the inferred gene products with characterized enzymes reveals that citP encodes the citrate permease P, citC the citrate ligase and citDEF the subunits of the citrate lyase of Leuconostoc. Moreover, it suggests that citM encodes a Leuconostoc malic enzyme. Analysis of citrate consumption by and citrate lyase activity of Lc. paramesenteroides J1[pCITJ1] showed that its citrate permease and its citrate lyase are induced by the presence of citrate in the growth medium. Southern blot analysis demonstrated that the citMCDEFGRP cluster is located in a plasmid.