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BIBTEX RIS

Utilização de um monitor de vídeo como fonte de alta tensão para eletroforese capilar

2006; Brazilian Chemical Society; Volume: 29; Issue: 6 Linguagem: Inglês

10.1590/s0100-40422006000600038

ISSN

1678-7064

Autores

José Alberto Fracassi da Silva, Leonardo Frasatto,

Tópico(s)

Microfluidic and Capillary Electrophoresis Applications

Resumo

CAPILLARY ELECTROPHORESIS.This article describes the use of a conventional CRT monitor as a high voltage power supply for capillary electrophoresis.With this monitor, a 23-kV high voltage with a ripple of 1.32% was observed.The reproducibility of the applied high voltage was evaluated by measuring the standard deviations of peak area and migration time for five consecutive injections of a test mixture containing potassium, sodium, and lithium cations at 50 μmol L -1 .The errors were about 2.5% and 0.6% for peak area and migration time, respectively.The maximum current tested was about 180 μA, which covers most capillary electrophoresis applications.This system has been successfully used for several months, maintaining the desired level of performance.

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