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Amino‐Acid Sequence of Toxin III of Naja haje

1975; Wiley; Volume: 58; Issue: 1 Linguagem: Inglês

10.1111/j.1432-1033.1975.tb02355.x

1432-1033

Charles Kopeyan, François Miranda, Hervé Rochat,

Protein Structure and Dynamics

The complete amino acid sequence of toxin III of Naja haje (72 residues) has been established mainly by use of a protein sequenator (identification of 70 residues). The two C‐terminal residues have been determined by digestion with carboxypeptidases A and B. Addition of succinylated protein or peptide greatly improved the performance of the sequenator for the Edman degradation of peptides: on one peptide (39 residues) degradation went to step 34 with a protein program and on two peptides (10 and 13 residues) degradation reached the last amino acid with a peptide program (use of dimethylbenzylamine). Amino acid analysis of tryptic peptides obtained by digestion of the C‐terminal cyanogen bromide peptide are in full agreement with the sequence established by automatic degradation. The sequence of toxin III of Naja haje is unique and is very similar to that of Naja nivea α (although there are 9 differences), of Naja melanoleuca b (11 differences) and also to that of Naja naja A (18 differences).