EasyProt — An easy-to-use graphical platform for proteomics data analysis
2012; Elsevier BV; Volume: 79; Linguagem: Inglês
10.1016/j.jprot.2012.12.012
ISSN1876-7737
AutoresFlorent Glück, Christine Hoogland, Paola Antinori, Xavier Robin, Frédéric Nikitin, Anne Zufferey, Carla Pasquarello, Vanessa Fétaud, Loı̈c Dayon, Markus Müller, Frédérique Lisacek, Laurent Geiser, Denis F. Hochstrasser, Jean-Charles Sánchez, Alexander Scherl,
Tópico(s)Metabolomics and Mass Spectrometry Studies
ResumoHigh throughput protein identification and quantification analysis based on mass spectrometry are fundamental steps in most proteomics projects. Here, we present EasyProt (available at http://easyprot.unige.ch), a new platform for mass spectrometry data processing, protein identification, quantification and unexpected post-translational modification characterization. EasyProt provides a fully integrated graphical experience to perform a large part of the proteomic data analysis workflow. Our goal was to develop a software platform that would fulfill the needs of scientists in the field, while emphasizing ease-of-use for non-bioinformatician users. Protein identification is based on OLAV scoring schemes and protein quantification is implemented for both, isobaric labeling and label-free methods. Additional features are available, such as peak list processing, isotopic correction, spectra filtering, charge-state deconvolution and spectra merging. To illustrate the EasyProt platform, we present two identification and quantification workflows based on isobaric tagging and label-free methods.
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