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The chemical ionization mass spectrometric analysis of phenylthiohydantoin and 2-anilino-5-thiazolinone amino acids obtained from the Edman degradation of proteins and peptides

1980; Elsevier BV; Volume: 107; Issue: 1 Linguagem: Inglês

10.1016/0003-2697(80)90504-7

1096-0309

Thomas Fairwell, H. Bryan Brewer,

Fluorine in Organic Chemistry

Abstract The development of accelerated automated methods for the sequence analysis of peptides and proteins has resulted in the need for an unambiguous and rapid method for identification of phenylthiohydantoin amino acids obtained from the Edman degradation. Chemical ionization mass spectrometry has been shown to be an extremely rapid, sensitive, and reliable method for the identification of all amino acid derivatives. Phenylthiohydantoin amino acids are identified by their protonated molecular ion or fragment ions which are characteristic of each amino acid derivative. This method can be used for identification of thiohydantoin as well as the thiazolinone derivatives of amino acids. Currently the chemical ionization method represents the fastest, easiest, and most versatile single method for identification of these amino acid derivatives. This method should facilitate the development of a totally automated system for the sequence analysis of peptides and proteins.