Transgenesis in large domestic species: future development for milk modification
1999; Elsevier BV; Volume: 39; Issue: 5-6 Linguagem: Inglês
10.1051/rnd
ISSN1297-9708
AutoresBelén Pintado, Alfonso Gutiérrez‐Adán,
Tópico(s)Virus-based gene therapy research
ResumoThe present review has two goals.First, to offer an overview of recent advances in the technical strategies applied to the production of transgenic large domestic animals, and second, to review how transgenic technology can be applied to the modification of milk composition.Transgenic sheep and cattle obtained through nuclear transfer are now a reality, opening up a means of ruminant transgenic production with an efficiency that entitles us to consider it a serious alternative to microin- jection.Nuclear transfer also consistently reduces the time needed to establish a transgenic production herd, and what is more important, it opens up the way to homologous recombination in large species, which at the moment is restricted to mice.Other interesting technological contributions have also taken place lately, some of them towards the modification of the male germ line, and oth- ers developing viral vectors with the ability to alter the genetic information of animals.The simpli- fication of the methodology and the consistent reduction of the time needed to carry out a trans- genic experiment will allow us to test several hypotheses directed at the modification of milk components.This may help towards the application of transgenic technology in the dairy industry, which unlike pharmaceutical companies, has been somehow reluctant over these approaches.© Inra/Elsevier, Paris transgenesis / milk / dairy industry / cattle Résumé ― Transgenèse chez les grands mammifères domestiques, développement à venir de * E-mail:
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