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Parallel stimulation of cyclic AMP accumulation and LH and FSH release by analogs of LH-RH in vitro

1974; Elsevier BV; Volume: 1; Issue: 1 Linguagem: Inglês

10.1016/0303-7207(74)90035-5

1872-8057

P Borgeat, Fernand Labrie, James E. Côté, F. Ruel, Andrew V. Schally, D.H. Coy, Esther J. Coy, Noboru Yanaihara,

Thyroid Disorders and Treatments

Dose-response studies with des-(pyro) Glu-LH-RH, [Phe3] LH-RH, [Phe5] LH-RH [0-methyl-Tyr5] LH-RH, [Ile7] LH-RH and des-Gly10-LH-RH-ethylamide show concomitant stimulation of adenohypophyseal adenosine 3',5'-monophosphate (cyclic AMP) concentration, and LH and FSH release in rat anterior pituitary gland in vitro. Des-His2-des-Gly10-LH-RH-ethylamide, and inhibitor of the response to LH-RH in vivo, had no effect on cyclic AMP accumulation or on LH release in vitro. The present finding of parallel changes of intracellular cyclic AMP levels and hormonal release with LH-RH analogs having a wide spectrum of biological activity adds strong support for a role of the cyclic nucleotide as mediator of the action of LH-RH in the anterior pituitary gland.