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Interaction of the restriction endonuclease Sca I with its substrates

1985; Oxford University Press; Volume: 13; Issue: 19 Linguagem: Inglês

10.1093/nar/13.19.7015

1362-4962

Keiko Kita, Nobutsugu Hiraoka, Fusao Kimizuka, Akira Ôbayashi, Hiroyuki Kojima, Hideo Takahashi, Hiuga Saito,

RNA and protein synthesis mechanisms

The kinetic constants of the site-specific endonuclease, ScaI, for various substrates were determined. We estimated Vmax and Km for octa-, deca-, dodeca-, and hexadecanucleotides and for plasmid pBR322 DNA. Vmax for these substrates were close, but Km were quite different (in decreasing order, octa- greater than deca-, dodeca-, hexadeca- greater than pBR322). The results were discussed with respect to the tertiary structure of substrate.