Carbon monoxide protects cardiomyogenic cells against ischemic death through L-type Ca2+ channel inhibition
2005; Elsevier BV; Volume: 334; Issue: 2 Linguagem: Inglês
10.1016/j.bbrc.2005.06.142
ISSN1090-2104
AutoresKoichi Uemura, Satomi Adachi‐Akahane, Kaori Shintani‐Ishida, Kenichi Yoshida,
Tópico(s)Neonatal Health and Biochemistry
ResumoCarbon monoxide (CO) is known to protect myocardial and vascular cells against injuries due to ischemia–reperfusion or inflammation. We showed that a Ca2+-dependent protease calpain promotes necrotic cell death of cardiomyocyte-derived H9c2 cells due to hypoxia through α-fodrin proteolysis. Here, we show that ischemia induces necrotic cell death, which is inhibited by either CO, extracellular Ca2+ deprivation or L-type Ca2+ channel blockers. A whole cell patch-clamp experiment supports that CO inhibits L-type Ca2+ channel mediated influx of Ca2+ and the ischemic death of H9c2 cells.
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