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Effect of Subcutaneous Injection and Oral Voluntary Ingestion of Buprenorphine on Post-Operative Serum Corticosterone Levels in Male Rats

2008; Karger Publishers; Volume: 41; Issue: 3 Linguagem: Inglês

10.1159/000142372

1421-9921

R. Goldkuhl, H.-E. Carlsson, Jann Hau, Klas S. P. Abelson,

Pharmacological Effects and Assays

<i>Background:</i> Adequate peri-operative analgesia may reduce post-operative stress response and improve recovery in laboratory animals. We have established a method involving repeated automated blood sampling, allowing quantification of serum corticosterone levels in rats for stress assessment without stress-inducing handling or restraint. In the present study, the effects of the commonly used route of buprenorphine administration (0.05 mg/kg injected subcutaneously) were compared with oral administration (0.4 mg/kg mixed with Nutella® and orally administered by voluntary ingestion) in male Sprague-Dawley rats. <i>Methods:</i> A catheter was placed in the jugular vein and attached to an Accusampler® for automated blood sampling. During 96 h after surgery, blood was collected at specified time points. Pre- and post-operative body weights and water consumption were registered. <i>Results:</i> Buprenorphine significantly suppressed levels of circulating corticosterone after the oral but not after the subcutaneous treatment. Both buprenorphine treatments had a positive impact on maintenance of body weight and water consumption, compared to the control group that received no buprenorphine. <i>Conclusion:</i> The present investigation suggests that oral voluntary ingestion ad libitum is an efficacious, convenient and non-invasive way of administering peri-operative buprenorphine to rats, as judged by corticosteroid response and effects on body weight and water consumption.