Portal de Periódicos da CAPES

Competitive protein binding assay for activin AEDF using follistatin determination of activin levels in human plasma

1992; Elsevier BV; Volume: 185; Issue: 3 Linguagem: Inglês

10.1016/0006-291x(92)91746-d

1090-2104

R Demura, Tomoharu Suzuki, SAEKO TAJIMA, SOEKO MITSUHASHI, E Odagiri, Yuzuru Eto, H Sugino, Hiroshi Demura,

Chronic Kidney Disease and Diabetes

A sensitive and specific protein binding assay for activin A/EDF (activin) was developed using follistatin as a binding protein and [125I] labelled activin as a tracer. As 50% acetonitrile (CH3CN) separated free and follistatin-bound activin, plasma pretreated with an equal volume of CH3CN was used as the assay sample and B/F separation was also done with 50% CH3CN. The recovery of the assay was 85.0% and its sensitivity was 0.5 ng/ml. Crossreactivity with inhibin A was 1.8%. The mean plasma level of follistatin-free activin in normal subjects was 1.3 +/- 0.7%. (M +/- SD) ng/ml. Plasma free activin levels were generally elevated in patients with chronic renal failure or hematological diseases associated with anemia.