Low‐conductance chloride channel activated by cAMP in the epithelial cell line T 84
1990; Wiley; Volume: 270; Issue: 1-2 Linguagem: Inglês
10.1016/0014-5793(90)81257-o
ISSN1873-3468
AutoresJoseph A. Tabcharani, W. Low, Dominique Élie, John W. Hanrahan,
Tópico(s)Neuroscience and Neuropharmacology Research
ResumoWe have studied the modulation and pharmacological properties of two anion channels in T 84 cells by recording single channel and transepithelial currents. One channel had an outwardly rectifying current‐voltage I/V curve, was rarely active in cell‐attached patches, and was unaffected by cAMP. The other channel had lower conductance (8.7 pS at 37°C) and a more ohmic I/V relationship. Exposure to cAMP increased the probability of observing low‐conductance channel activity in cell‐attached patches > 6‐fold. Extracellular DIDS (4,4'‐diisothiocyanatostilbene‐2,2'‐disulfonic acid) or IAA‐94 (an indanyloxyacetic acid) inhibited the outward rectifier but did not affect the low‐conductance channel or cAMP‐stimulated transepithelial current. These results suggest the low‐conductance Cl channel may contribute to apical membrane conductance during cAMP‐stimulated secretion.
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