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Effects of Hemodialysis on Testicular Volume and Oxidative Stress in Humans

2008; Lippincott Williams & Wilkins; Volume: 180; Issue: 2 Linguagem: Inglês

10.1016/j.juro.2008.04.010

1527-3792

Koji Shiraishi, Tomoyuki Shimabukuro, Katsusuke Naito,

Hormonal and reproductive studies

No AccessJournal of UrologyAdult Urology1 Aug 2008Effects of Hemodialysis on Testicular Volume and Oxidative Stress in Humansis accompanied byEnhancement of Human Sperm Motility by Trophinin Binding PeptideThe Vasodilator Effect of Testosterone on the Human Internal Spermatic Vein and its Relation to Varicocele Grade Koji Shiraishi, Tomoyuki Shimabukuro, and Katsusuke Naito Koji ShiraishiKoji Shiraishi Department of Urology, Ube Industries Central Hospital, Ube, Yamaguchi, Japan , Tomoyuki ShimabukuroTomoyuki Shimabukuro Department of Urology, Ube Industries Central Hospital, Ube, Yamaguchi, Japan , and Katsusuke NaitoKatsusuke Naito Department of Urology, Graduate School of Medicine, Yamaguchi University, Ube, Yamaguchi, Japan View All Author Informationhttps://doi.org/10.1016/j.juro.2008.04.010AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Male infertility is a serious problem in patients on hemodialysis. Our understanding is that end stage renal disease or hemodialysis causes poor semen quality but the mechanism leading to impaired spermatogenesis is largely unknown. Materials and Methods: Testicular volume in 120 patients on maintenance hemodialysis was compared with that in age matched healthy controls. Volume was correlated with clinical findings. In 10 testicular biopsy specimens from patients on hemodialysis who visited our infertility clinic Western blotting was performed to examine the generation of 4-HNE modified proteins, which are markers of oxidative stress, and the expression of proliferating cell nuclear antigen. Interstitial fibrosis was determined by Masson's trichrome staining. Results: Mean bilateral testicular volume in patients on hemodialysis was significantly smaller than that in healthy controls (31.7 vs 36.4 ml, p <0.01) in a hemodialysis duration dependent manner (r = −0.32, p <0.01). The increase in serum ferritin correlated inversely with testicular volume (r = −0.25, p <0.01). The generation of 4-HNE modified proteins was significantly increased 3.1-fold in patients on hemodialysis, following the 60% decreased expression of proliferating cell nuclear antigen. Quantitative analysis of Masson's trichrome staining revealed increased interstitial fibrosis in patients on hemodialysis compared with that in controls (41.5% vs 14.8%, p <0.01). Serum ferritin, proliferating cell nuclear antigen expression and interstitial fibrosis correlated with the generation of 4-HNE modified proteins (p <0.05). Conclusions: Testicular volume, which is a parameter of spermatogenesis, is impaired in patients on hemodialysis and oxidative stress is considered to be involved in the process. Serum ferritin is a useful parameter for predicting oxidative stress in the testis. 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Google Scholar © 2008 by American Urological AssociationFiguresReferencesRelatedDetailsRelated articlesJournal of Urology13 Jun 2008Enhancement of Human Sperm Motility by Trophinin Binding PeptideJournal of Urology13 Jun 2008The Vasodilator Effect of Testosterone on the Human Internal Spermatic Vein and its Relation to Varicocele Grade Volume 180Issue 2August 2008Page: 644-650 Advertisement Copyright & Permissions© 2008 by American Urological AssociationKeywordsrenal dialysistestismaleinfertilityoxidative stressspermatogenesisMetricsAuthor Information Koji Shiraishi Department of Urology, Ube Industries Central Hospital, Ube, Yamaguchi, Japan More articles by this author Tomoyuki Shimabukuro Department of Urology, Ube Industries Central Hospital, Ube, Yamaguchi, Japan More articles by this author Katsusuke Naito Department of Urology, Graduate School of Medicine, Yamaguchi University, Ube, Yamaguchi, Japan More articles by this author Expand All Advertisement PDF downloadLoading ...