Colony Size and Giant Cell Formation from Mammalian Cells Exposed to 1 MHz Ultrasound
1977; Radiation Research Society; Volume: 71; Issue: 3 Linguagem: Inglês
10.2307/3574630
ISSN1938-5404
AutoresMorton W. Miller, Victor Ciaravino, Gary E. Kaufman,
Tópico(s)Electromagnetic Fields and Biological Effects
ResumoChinese hamster ovary (CHO) cells were exposed to continuous wave 1 MHz, $5 {\rm W}/{\rm cm}^{2}$ ultrasound for 5 min, plated, and allowed to grow for 7 days; colonies were then scored for size and for the presence of giant cells. Sonicated colonies contained fewer cells than controls by a factor 0.53 (P < 0.001), but contained more giant cells than sham controls by a factor of 1.30 (P < 0.001). On a per cell basis the frequency of giant cells was 2.47 times higher in the colonies arising from sonicated cells. Characteristics of giant cells varied; all were abnormally large cells with one large nucleus or several smaller nuclei and/or protoplasmic bridges.
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