Determination of Naproxen Using DBS: Evaluation & Pharmacokinetic Comparison of Human Plasma Versus Human Blood DBS
2010; Future Science Ltd; Volume: 2; Issue: 8 Linguagem: Inglês
10.4155/bio.10.51
ISSN1757-6199
AutoresNikolay Youhnovski, Josée Michon, Sylvain Latour, Jean‐Nicholas Mess, Annik Bergeron, Milton Furtado, Marianne Rufiange, Pascal Guibord, Marc Lefebvre, Robert B. MacArthur, Fabio Garofolo,
Tópico(s)Microfluidic and Capillary Electrophoresis Applications
ResumoDried blood spots (DBS) sampling is a well-known technology for qualitative determination such as DNA analysis and screening of newborn metabolic disorders. The scientific community has recently expressed interest in applying the DBS technique for quantitative determination of drugs in biological fluid.Two new bioanalytical assays were developed and validated for the determination of naproxen in human plasma and in DBS samples using liquid chromatography coupled with tandem MS. Furthermore, plasma and DBS clinical samples were collected from four subjects enrolled as part of a bioequivalence study. Concentration data for plasma and DBS samples were determined and pharmacokinetic (PK) profiles in plasma and in DBS samples were compared.A strong correlation between PK data obtained by the DBS and conventional plasma method was observed, which makes DBS a valuable technique for further naproxen bioavailability and PK investigations and studies.
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