Produção Nacional
Revisado por pares
Detection of canine distemper virus by reverse transcriptase-polymerase chain reaction in the urine of dogs with clinical signs of distemper encephalitis
2005; Elsevier BV; Volume: 80; Issue: 1 Linguagem: Inglês
10.1016/j.rvsc.2005.03.002
ISSN1532-2661
AutoresTaís B. Saito, Amauri Alcindo Alfieri, Sheila Rezler Wosiacki, Fábio Juliano Negrão, Helio S. Autran de Morais, Alice Fernandes Alfieri,
Tópico(s)Animal Disease Management and Epidemiology
ResumoIn a prospective study we evaluated the use of the reverse transcriptase-polymerase chain reaction (RT-PCR) in urine samples to diagnose canine distemper virus in dogs with progressive neurological disease. A fragment of the nucleoprotein gene of canine distemper virus was amplified from the urine of 22 distemper dogs. The body fluids and leukocytes of 12 asymptomatic dogs were RT-PCR negative. RT-PCR of urine samples was more sensitive than serum and leukocytes and at least as sensitive as cerebrospinal fluid to screen for distemper in dogs with neurological signs and extraneural systemic signs.
Referência(s)