The inducible trimethylamine N-oxide reductase of Escherichia coli K12: its localization and inducers
1989; Elsevier BV; Volume: 999; Issue: 2 Linguagem: Inglês
10.1016/0167-4838(89)90220-3
ISSN1878-1454
AutoresAndré Silvestro, Janine Pommier, Marie‐Claire Pascal, Gérard Giordano,
Tópico(s)Hemoglobin structure and function
ResumoWe used an anti-trimethylamine-N-oxide reductase (EC 1.6.6.9) serum and different immunological techniques (Ouchterlony, rocket immunoelectrophoresis, immunoblotting) to show that dimethylsulphoxide (DMSO), tetrahydrothiophene 1-oxide (THTO) and pyridine N-oxide (PNO) were effective inducers of the inducible form of trimethylamine N-oxide reductase. We confirmed this genetically and biochemically using a strain in which phage MudII 1734 carrying lacZ was inserted into torA, the structural gene for inducible trimethylamine-N-oxide reductase. By subcellular fractionation and quantitation with rocket immunoelectrophoresis, we showed that the enzyme was principally localized in the periplasmic fraction. Constitutive trimethylamine-N-oxide reductase was localized in the membrane fraction and, like the inducible enzyme showed a broad specificity with respect to various compounds such as DMSO, THTO and PNO. Apart from their immunological properties, the two enzymes could be clearly differentiated by their temperature stability.
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